Functional expression of the reverse transcriptase αβ heterodimer from avian myeloblastosis virus in Escherichia coli

Yuriko Makino; Hiroshi Sato; Atsushi Noguchi; Teruhiko Ide; Rie Yatsunami; Satoshi Nakamura

doi:10.1093/bbb/zbab057

Functional expression of the reverse transcriptase αβ heterodimer from avian myeloblastosis virus in Escherichia coli

Biosci Biotechnol Biochem. 2021 May 25;85(6):1464-1467. doi: 10.1093/bbb/zbab057.

Authors

Yuriko Makino^{1

2}, Hiroshi Sato³, Atsushi Noguchi², Teruhiko Ide², Rie Yatsunami¹, Satoshi Nakamura^{1

4}

Affiliations

¹ School of Life Science and Technology, Tokyo Institute of Technology, Yokohama, Japan.
² Life Science Research Laboratory, Tosoh Corp., Ayase, Kanagawa, Japan.
³ Bioscience Division, Tosoh Corp., Minato-ku, Tokyo, Japan.
⁴ National Institute of Technology, Numazu College, Numazu, Shizuoka, Japan.

PMID: 33826692
DOI: 10.1093/bbb/zbab057

Abstract

The α subunit of avian myeloblastosis virus reverse transcriptase (AMV-RT) is generated from the β-subunit by proteolysis, and the αβ heterodimer represents the active form. The codon-optimized gene was expressed in Escherichia coli, and an active αβ heterodimer was generated. The RNA amplification activity of the purified recombinant AMV-RT αβ heterodimer was similar to that of the native one.

Keywords: TRC reaction; avian myeloblastosis virus; reverse transcriptase; αβ heterodimer.

MeSH terms

Avian Myeloblastosis Virus / enzymology*
Escherichia coli / genetics*
Gene Expression
Protein Multimerization*
Protein Structure, Quaternary
RNA-Directed DNA Polymerase / chemistry*
RNA-Directed DNA Polymerase / genetics
RNA-Directed DNA Polymerase / metabolism*

Substances

RNA-Directed DNA Polymerase