Efflux and assimilation of xylem-transported CO2 in stems and leaves of tree species with different wood anatomy

Abstract

Determining the fate of CO₂ respired in woody tissues is necessary to understand plant respiratory physiology and to evaluate CO₂ recycling mechanisms. An aqueous ¹³ C-enriched CO₂ solution was infused into the stem of 3-4 m tall trees to estimate efflux and assimilation of xylem-transported CO₂ via cavity ring-down laser spectroscopy and isotope ratio mass spectrometry, respectively. Different tree locations (lower stem, upper stem and leafy shoots) and tissues (xylem, bark and leaves) were monitored in species with tracheid, diffuse- and ring-porous wood anatomy (cedar, maple and oak, respectively). Radial xylem CO₂ diffusivity and xylem [CO₂ ] were lower in cedar relative to maple and oak trees, thereby limiting label diffusion. Part of the labeled ¹³ CO₂ was assimilated in cedar (8.7%) and oak (20.6%) trees, mostly in xylem and bark tissues of the stem, while limited solution uptake in maple trees hindered the detection of label assimilation. Little label reached foliar tissues, suggesting substantial label loss along the stem-branch transition following reductions in the radial diffusive pathway. Differences in respiration rates and radial xylem CO₂ diffusivity (lower in conifer relative to angiosperm species) might reconcile discrepancies in efflux and assimilation of xylem-transported CO₂ so far observed between taxonomic clades.

Keywords: 13C-CO2 labelling; CO2 diffusion; CO2 recycling; carbon allocation; plant respiration; stem CO2 efflux; woody tissue photosynthesis; xylem CO2 transport.