A genetically encoded sensor for measuring serotonin dynamics

Jinxia Wan; Wanling Peng; Xuelin Li; Tongrui Qian; Kun Song; Jianzhi Zeng; Fei Deng; Suyu Hao; Jiesi Feng; Peng Zhang; Yajun Zhang; Jing Zou; Sunlei Pan; Mimi Shin; B Jill Venton; J Julius Zhu; Miao Jing; Min Xu; Yulong Li

doi:10.1038/s41593-021-00823-7

A genetically encoded sensor for measuring serotonin dynamics

Nat Neurosci. 2021 May;24(5):746-752. doi: 10.1038/s41593-021-00823-7. Epub 2021 Apr 5.

Authors

Jinxia Wan^{1

2}, Wanling Peng³, Xuelin Li^{1

2}, Tongrui Qian^{1

2}, Kun Song³, Jianzhi Zeng^{1

2

4}, Fei Deng^{1

2}, Suyu Hao^{1

2}, Jiesi Feng^{1

2

4}, Peng Zhang⁵, Yajun Zhang⁵, Jing Zou^{1

2

6}, Sunlei Pan^{1

2

4}, Mimi Shin⁷, B Jill Venton⁷, J Julius Zhu⁵, Miao Jing⁸, Min Xu³, Yulong Li^{9

10

11}

Affiliations

¹ State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China.
² PKU-IDG/McGovern Institute for Brain Research, Beijing, China.
³ Institute of Neuroscience, State Key Laboratory of Neuroscience, CAS Center for Excellence in Brain Science and Intelligence Technology, Chinese Academy of Sciences, Shanghai, China.
⁴ Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
⁵ Department of Pharmacology, University of Virginia School of Medicine, Charlottesville, VA, USA.
⁶ Department of Biological Sciences, Neurobiology Section, University of Southern California, Los Angeles, CA, USA.
⁷ Department of Chemistry, University of Virginia, Charlottesville, VA, USA.
⁸ Chinese Institute for Brain Research, Beijing, China.
⁹ State Key Laboratory of Membrane Biology, Peking University School of Life Sciences, Beijing, China. yulongli@pku.edu.cn.
¹⁰ PKU-IDG/McGovern Institute for Brain Research, Beijing, China. yulongli@pku.edu.cn.
¹¹ Peking-Tsinghua Center for Life Sciences, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China. yulongli@pku.edu.cn.

Abstract

Serotonin (5-HT) is a phylogenetically conserved monoamine neurotransmitter modulating important processes in the brain. To directly visualize the release of 5-HT, we developed a genetically encoded G-protein-coupled receptor (GPCR)-activation-based 5-HT (GRAB_5-HT) sensor with high sensitivity, high selectivity, subsecond kinetics and subcellular resolution. GRAB_5-HT detects 5-HT release in multiple physiological and pathological conditions in both flies and mice and provides new insights into the dynamics and mechanisms of 5-HT signaling.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Female
HEK293 Cells
Humans
Male
Mice
Neurons / metabolism*
Rats
Receptors, G-Protein-Coupled / metabolism*
Serotonergic Neurons / metabolism*
Serotonin / metabolism*
Signal Transduction / physiology

Substances

Receptors, G-Protein-Coupled
Serotonin

Abstract

Publication types

MeSH terms

Substances

Grants and funding