Succession of the microbial communities and function prediction during short-term peach sawdust-based composting

Yu-Xin Guo; Qing-Jun Chen; Yong Qin; Ya-Ru Yang; Qi-Zhi Yang; Yue-Xing Wang; Zi-An Cheng; Na Cao; Guo-Qing Zhang

doi:10.1016/j.biortech.2021.125079

Succession of the microbial communities and function prediction during short-term peach sawdust-based composting

Bioresour Technol. 2021 Jul:332:125079. doi: 10.1016/j.biortech.2021.125079. Epub 2021 Mar 30.

Authors

Yu-Xin Guo¹, Qing-Jun Chen², Yong Qin³, Ya-Ru Yang², Qi-Zhi Yang², Yue-Xing Wang², Zi-An Cheng², Na Cao², Guo-Qing Zhang⁴

Affiliations

¹ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China; College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China.
² Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China.
³ College of Forestry and Horticulture, Xinjiang Agricultural University, Urumqi 830052, China.
⁴ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Key Laboratory for Agricultural Application and New Technique, College of Plant Science and Technology, Beijing University of Agriculture, Beijing 102206, China. Electronic address: zhanggq1001@bua.edu.cn.

PMID: 33813177
DOI: 10.1016/j.biortech.2021.125079

Abstract

Short-term composting of raw materials for preparing oyster mushroom cultivation media is widely used in China, and its microbial mechanism needs to be further studied. 11-days' peach sawdust-based composting was performed to evaluate material conversion and microbial succession using physicochemical analysis and 16S rRNA and ITS sequencing. Composting bacteria demonstrated much higher abundance than fungi. Firmicutes, Actinobacteriota, and Proteobacteria were the dominant bacterial phyla, while most of fungal species belonged to Ascomycota. Moisture was the key factor at the beginning, while total nitrogen, temperature, and lignin became main influencing factors for composting maturity. Actinobacteriota, Firmicutes, and Proteobacteria of bacterial phyla, Eurotiomycetes and Sordariomycetes of fungal classes involved in lignocellulosic degradation. Bacterial function prediction analysis showed that carbohydrate metabolism and amino acid metabolism were the main metabolic pathways. These results confer a better understanding of material and microbial succession during short-term composting and also provide valuable utilization in mushroom industry.

Keywords: Composting; Function prediction; Lignocellulosic degradation; Microbial community; Peach sawdust.

MeSH terms

China
Composting*
Manure
Microbiota*
Prunus persica*
RNA, Ribosomal, 16S / genetics
Soil

Substances

Manure
RNA, Ribosomal, 16S
Soil