Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9 genome editing technology

Mingze Yao; Pengfei Su; Zhengfeng Li; Xiaogang Cui; Qian Yang; Xiaoyu Xing; Yu Lei; Mengqiao Lian; Tinglin Ren; Xiedong Wang; Yaowu Zheng; Changxin Wu

doi:10.1016/j.scr.2021.102236

Knockout of Dip2c in murine ES cell line IBMSe001-B-1 by CRISPR/Cas9 genome editing technology

Stem Cell Res. 2021 May:53:102236. doi: 10.1016/j.scr.2021.102236. Epub 2021 Feb 16.

Authors

Mingze Yao¹, Pengfei Su², Zhengfeng Li³, Xiaogang Cui², Qian Yang², Xiaoyu Xing², Yu Lei², Mengqiao Lian², Tinglin Ren², Xiedong Wang², Yaowu Zheng⁴, Changxin Wu⁵

Affiliations

¹ Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China. Electronic address: yaomz@sxu.edu.cn.
² Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China.
³ Faculty of Health Sciences, University of Macau, Avenida da Universidade, Taipa, Macau, China.
⁴ Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China; Transgenic Research Center, Northeast Normal University, Changchun, China. Electronic address: zhengyw442@nenu.edu.cn.
⁵ Institutes of Biomedical Sciences, Shanxi University, No. 92 Wucheng Road, 030006 Taiyuan, China. Electronic address: cxw20@sxu.edu.cn.

PMID: 33813174
DOI: 10.1016/j.scr.2021.102236

Abstract

DIP2 protein contains three members: DIP2A, DIP2B and DIP2C, and are broadly expressed in the nervous system from Drosophila to human during embryonic development. Dip2c gene-associated mutations have been reported in tumors and neuronal diseases. However, the role ofDip2cin the context of mouse embryonic stem (mES) cells has not been explored.To investigate the biological function of Dip2c during early embryo development, we generated Dip2c^-/- mES line using a CRISPR/Cas9 system. This cell line has contributed to further investigation of molecular mechanism of Dip2c during cell differentiation, as well as a cell model for screening for neurogenic drug and cancer clinical cure.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CRISPR-Cas Systems* / genetics
Cell Line
Female
Gene Editing*
Mice
Mice, Knockout
Pregnancy
Technology