Objective: The present study was to evaluate the anti-tumor effects of acidic RNA protein complex (FA-2-b-β) extracted from the wild edible Qinba mushroom in inducing of apoptosis and immunoregulation of tumor cell.
Methods: Cell proliferation inducing rate of FA-2-b-β to K562 cell was measured using CCK-8. Apoptosis rate was detected by using flow cytometry. Chronic myeloid leukemia model was developed by tail vein injection/subcutaneous inoculation of K562 cells in NCG mice. The tumor burden of mice was observed. The general condition of the mice was monitored twice daily. The peripherivcal full blood counts of mice was tested daily. RT-qPCR and Western blot was FA-2-b-β performed to determine involvement of apoptotic-related gene and protenin, Immunofluorescence and immunohistochemistry was used to detected the expression of CD3, CD4 and CD8.
Results: The proliferation and apoptosis of K562 cell could be inhibitied and induced by FA-2-b-β, there was 100% successful in the tumor formation in vivo, after treated by drug for 21 days there were significantly increased peripheral leucocytes, but decreased hemoglobin of mice treated by FA-2-b-β as compared with those in control group. The CD3, CD4 and CD8 showed positive in mice, and the propotation was imbalance, but it showed reserved after treated by FA-2-b-β.
Conclusion: FA-2-b-β is strong anti-leukemia effect in vitro and in vivo, suggesting the traditional Chinese medicine maybe contribute to the anti-cancer and immunoregulation research.
题目: 秦巴硒菇提取物对NCG小鼠慢性髓系白血病模型体内抗肿瘤的初步研究.
目的: 验证秦巴硒菇提取物酸性RNA蛋白复合物FA-2-b-β诱导肿瘤细胞凋亡及免疫调节的作用.
方法: 采用CCK-8法计算FA-2-b-β对K562细胞的增殖抑制率，流式细胞术检测细胞凋亡率。通过尾静脉/皮下注射K562细胞构建慢性髓系白血病模型，观察小鼠成瘤负荷，每天2次观察小鼠一般情况，定期检测小鼠外周血细胞数。RT-qPCR及Western blot检测FA-2-b-β作用于白血病小鼠后相关凋亡基因/蛋白的表达；免疫荧光和免疫组织化学检测CD3、CD4、CD8的表达.
结果: FA-2-b-β抑制K562细胞的增殖并诱导其凋亡，模型小鼠成瘤率100%，药物治疗21天后，与对照组相比，外周血白细胞数明显升高，血红蛋白降低；免疫荧光及免疫组化结果显示CD3、CD4、CD8阳性，比例失调，治疗后各项指标有所改善.
结论: 初步验证秦巴硒菇提取物FA-2-b-β具有诱导肿瘤细胞凋亡及免疫调节作用.