Derivation of a human induced pluripotent stem cell (hiPSC) line (PUMCSMi001-A) from peripheral blood mononuclear cells of one healthy female donor

Mingxia Du; Lulu Li; Yaoda Hu; Huan Mi; Tianran Wang

doi:10.1016/j.scr.2021.102312

Derivation of a human induced pluripotent stem cell (hiPSC) line (PUMCSMi001-A) from peripheral blood mononuclear cells of one healthy female donor

Stem Cell Res. 2021 May:53:102312. doi: 10.1016/j.scr.2021.102312. Epub 2021 Mar 24.

Authors

Mingxia Du¹, Lulu Li², Yaoda Hu³, Huan Mi³, Tianran Wang⁴

Affiliations

¹ State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China. Electronic address: dumingxia@ibms.pumc.edu.cn.
² Department of Newborn Screening Center, Beijing Obstetrics and Gynecology Hospital, Capital Medical University, Beijing Maternal and Child Health Care Hospital, BeiJing, China.
³ State Key Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, Beijing, China.
⁴ School of Life Sciences, Beijing University of Chinese Medicine, Beijing, China. Electronic address: wangtianran@bucm.edu.cn.

PMID: 33812336
DOI: 10.1016/j.scr.2021.102312

Abstract

Human induced pluripotent stem cells (hiPSCs) are valuable models for studying the molecular pathogenesis of inherited diseases and key tools for cell therapy. We derived peripheral blood mononuclear cells (PBMCs) isolated from a 30-year-old healthy female donor using integration-free Sendai virus to generate a hiPSC line, PUMCSMi001-A, which can serve as a control for the studies of pathogenesis of cardiovascular disease and drug screening. Quality assessment confirmed that the cell line PUMCSMi001-A expressed pluripotency genes, had normal karyotype and differentiation potential into three layers in vivo.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Cell Differentiation
Cellular Reprogramming
Female
Humans
Induced Pluripotent Stem Cells*
Leukocytes, Mononuclear
Sendai virus / genetics