Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia

Ivan S Kholodilov; Oxana A Belova; Evgeny S Morozkin; Alexander G Litov; Anna Y Ivannikova; Marat T Makenov; Alexey M Shchetinin; Sergey V Aibulatov; Galina K Bazarova; Lesley Bell-Sakyi; Liubov A Bespyatova; Sergey V Bugmyrin; Nikita Chernetsov; Liubov L Chernokhaeva; Larissa V Gmyl; Anna N Khaisarova; Alexei V Khalin; Alexander S Klimentov; Irina V Kovalchuk; Svetlana V Luchinina; Sergey G Medvedev; Alexander A Nafeev; Natalia D Oorzhak; Elena V Panjukova; Alexandra E Polienko; Kristina A Purmak; Evgeniya N Romanenko; Evgeniy N Rozhdestvenskiy; Anna A Saryglar; Anton F Shamsutdinov; Nataliya I Solomashchenko; Vladimir A Trifonov; Evgenii G Volchev; Pavel G Vovkotech; Alexander S Yakovlev; Olga B Zhurenkova; Vladimir A Gushchin; Lyudmila S Karan; Galina G Karganova

doi:10.3390/v13030458

Geographical and Tick-Dependent Distribution of Flavi-Like Alongshan and Yanggou Tick Viruses in Russia

Viruses. 2021 Mar 11;13(3):458. doi: 10.3390/v13030458.

Authors

Ivan S Kholodilov¹, Oxana A Belova¹, Evgeny S Morozkin², Alexander G Litov¹, Anna Y Ivannikova¹, Marat T Makenov², Alexey M Shchetinin³, Sergey V Aibulatov⁴, Galina K Bazarova⁵, Lesley Bell-Sakyi⁶, Liubov A Bespyatova⁷, Sergey V Bugmyrin⁷, Nikita Chernetsov^{8

9}, Liubov L Chernokhaeva¹, Larissa V Gmyl¹, Anna N Khaisarova¹⁰, Alexei V Khalin⁴, Alexander S Klimentov^{11

12}, Irina V Kovalchuk^{13

14}, Svetlana V Luchinina¹⁵, Sergey G Medvedev⁴, Alexander A Nafeev¹⁰, Natalia D Oorzhak¹⁶, Elena V Panjukova¹⁷, Alexandra E Polienko¹, Kristina A Purmak¹⁸, Evgeniya N Romanenko¹⁸, Evgeniy N Rozhdestvenskiy¹⁹, Anna A Saryglar¹⁶, Anton F Shamsutdinov²⁰, Nataliya I Solomashchenko^{13

18}, Vladimir A Trifonov^{20

21}, Evgenii G Volchev²², Pavel G Vovkotech¹⁰, Alexander S Yakovlev¹, Olga B Zhurenkova², Vladimir A Gushchin^{3

23}, Lyudmila S Karan², Galina G Karganova^{1

24}

Affiliations

¹ Laboratory of Biology of Arboviruses, "Chumakov Institute of Poliomyelitis and Viral Encephalitides" FSBSI "Chumakov FSC R&D IBP RAS", 108819 Moscow, Russia.
² Department of Molecular Diagnostics and Epidemiology, Central Research Institute of Epidemiology, 111123 Moscow, Russia.
³ Pathogenic Microorganisms Variability Laboratory, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia.
⁴ Laboratory of Parasitic Arthropods, Zoological Institute, Russian Academy of Sciences, 199034 St. Petersburg, Russia.
⁵ Laboratory of Bacteriology, Altai Antiplague Station of Rospotrebnadzor, 649000 Gorno-Altaisk, Russia.
⁶ Department of Infection Biology and Microbiomes, Institute of Infection, Veterinary and Ecological Sciences, University of Liverpool, Liverpool L3 5RF, UK.
⁷ Laboratory for Animal and Plant Parasitology, Institute of Biology of Karelian Research Centre, Russian Academy of Sciences (IB KarRC RAS), 185910 Petrozavodsk, Russia.
⁸ Laboratory of Ornithology, Zoological Institute, Russian Academy of Sciences, 199034 St. Petersburg, Russia.
⁹ Department of Vertebrate Zoology, St. Petersburg State University, 199034 St. Petersburg, Russia.
¹⁰ Center for Hygiene and Epidemiology in the Ulyanovsk Region, 432005 Ulyanovsk, Russia.
¹¹ Laboratory of Biochemistry, "Chumakov Institute of Poliomyelitis and Viral Encephalitides" FSBSI "Chumakov FSC R&D IBP RAS", 108819 Moscow, Russia.
¹² Laboratory of Biology and Indication of Arboviruses, Department Ivanovsky Institute of Virology, Gamaleya Federal Research Centre for Epidemiology and Microbiology, Ministry of Health of the Russian Federation, 123098 Moscow, Russia.
¹³ Office of Rospotrebnadzor in the Stavropol Territory, 355008 Stavropol, Russia.
¹⁴ Stavropol State Medical University, 355017 Stavropol, Russia.
¹⁵ Office of Rospotrebnadzor in the Chelyabinsk Region, 454092 Chelyabinsk, Russia.
¹⁶ Infectious Disease Hospital, 667003 Kyzyl, Russia.
¹⁷ Institute of Biology, Komi Science Center, Ural Branch of Russian Academy of Sciences, 167982 Syktyvkar, Russia.
¹⁸ FBIH "Center for Hygiene and Epidemiology in the Stavropol kray", 355008 Stavropol, Russia.
¹⁹ Director, Altai Antiplague Station of Rospotrebnadzor, 649000 Gorno-Altaisk, Russia.
²⁰ Kazan Scientific Research Institute of Epidemiology and Microbiology of Rospotrebnadzor, 420015 Kazan, Russia.
²¹ Kazan State Medical Academy-Branch Campus of the FSBEI FPE «Russian Medical Academy of Continuous Postgraduate Education» of the Ministry of Healthcare of the Russian Federation, 420012 Kazan, Russia.
²² Institute of Living Systems Immanuel Kant Baltic Federal University, 236041 Kaliningrad, Russia.
²³ Faculty of Biology, Lomonosov MSU, 119991 Moscow, Russia.
²⁴ Institute for Translational Medicine and Biotechnology, Sechenov University, 119146 Moscow, Russia.

Abstract

The genus Flavivirus includes related, unclassified segmented flavi-like viruses, two segments of which have homology with flavivirus RNA-dependent RNA polymerase NS5 and RNA helicase-protease NS3. This group includes such viruses as Jingmen tick virus, Alongshan virus, Yanggou tick virus and others. We detected the Yanggou tick virus in Dermacentor nuttalli and Dermacentor marginatus ticks in two neighbouring regions of Russia. The virus prevalence ranged from 0.5% to 8.0%. We detected RNA of the Alongshan virus in 44 individuals or pools of various tick species in eight regions of Russia. The virus prevalence ranged from 0.6% to 7.8%. We demonstrated the successful replication of the Yanggou tick virus and Alongshan virus in IRE/CTVM19 and HAE/CTVM8 tick cell lines without a cytopathic effect. According to the phylogenetic analysis, we divided the Alongshan virus into two groups: an Ixodes persulcatus group and an Ixodes ricinus group. In addition, the I. persulcatus group can be divided into European and Asian subgroups. We found amino acid signatures specific to the I. ricinus and I. persulcatus groups and also distinguished between the European and Asian subgroups of the I. persulcatus group.

Keywords: Alongshan virus; Flavivirus; Jingmen tick virus; Yanggou tick virus; amino acid substitutions; flavi-like virus; segmented virus; tick cell line.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Substitution / genetics
Animals
Arachnid Vectors / virology
Cell Line
Culicidae / virology
Dermacentor / virology*
Flaviviridae / genetics*
Flaviviridae / isolation & purification
Flaviviridae Infections / epidemiology*
Ixodes / virology*
Phylogeny
RNA Helicases / genetics
RNA, Viral / genetics
Russia / epidemiology
Serine Endopeptidases / genetics
Viral Nonstructural Proteins / genetics*

Substances

NS3 protein, flavivirus
NS5 protein, flavivirus
RNA, Viral
Viral Nonstructural Proteins
Serine Endopeptidases
RNA Helicases

Grants and funding

BB/P024270/1/BB_/Biotechnology and Biological Sciences Research Council/United Kingdom