Assembling cyanidin-3-O-glucoside by using low-viscosity alginate to improve its in vitro bioaccessibility and in vivo bioavailability

Chao Zou; Ling Huang; Donghui Li; Yu Ma; Yixiang Liu; Yanbo Wang; Min-Jie Cao; Guang-Ming Liu; Lechang Sun

doi:10.1016/j.foodchem.2021.129681

Assembling cyanidin-3-O-glucoside by using low-viscosity alginate to improve its in vitro bioaccessibility and in vivo bioavailability

Food Chem. 2021 Sep 1:355:129681. doi: 10.1016/j.foodchem.2021.129681. Epub 2021 Mar 25.

Authors

Chao Zou¹, Ling Huang¹, Donghui Li¹, Yu Ma¹, Yixiang Liu², Yanbo Wang³, Min-Jie Cao⁴, Guang-Ming Liu⁵, Lechang Sun¹

Affiliations

¹ College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China.
² College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China; Xiamen Key Laboratory of Marine Functional Food, Xiamen, Fujian 361021, China; National & Local Joint Engineering Research Center of Deep Processing Technology for Aquatic Products, Xiamen, Fujian 361021, China. Electronic address: lyxjmu@jmu.edu.cn.
³ College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China; School of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou, China.
⁴ College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China; National & Local Joint Engineering Research Center of Deep Processing Technology for Aquatic Products, Xiamen, Fujian 361021, China.
⁵ College of Food and Biological Engineering, Jimei University, Xiamen, Fujian 361021, China; Xiamen Key Laboratory of Marine Functional Food, Xiamen, Fujian 361021, China.

PMID: 33799247
DOI: 10.1016/j.foodchem.2021.129681

Abstract

In this work, an enteric soluble alginate was proposed to improve the absorption efficiency of cyanidin-3-O-glucoside (C3G) through molecular self-assembly. Under the optimized conditions, the obtained low-viscosity alginate (LVA) was released completely during the simulated gastrointestinal digestion and an LVA-C3G complex with 84.2% binding efficiency was acquired. Fourier transform infrared spectroscopy displayed that the characteristic spectrum of C3G had disappeared after the LVA conjugation. Furthermore, based on the analysis of scanning electron microscopy and differential scanning calorimetry, a porous network structure and the shifted endothermic peak in the thermograms were observed, further confirming the formation of a complex between LVA and C3G. The results of simulated gastrointestinal digestion reveal that the LVA assembly significantly (p < 0.05) improved the bioaccessibility of C3G. Correspondingly, the C3G level in mouse plasma was increased by 27.4% in the C3G-LVA group. This suggests the suitability of LVA as an oral delivery vehicle for dietary anthocyanins.

Keywords: Alginate; Bioaccessibility; Cyanidin-3-O-glucoside; Gastrointestinal digestion; Molecular docking; Molecular self-assembly.

MeSH terms

Alginates / chemistry*
Animals
Anthocyanins / chemistry*
Anthocyanins / metabolism
Anthocyanins / pharmacokinetics*
Biological Availability
Digestion
Mice
Viscosity

Substances

Alginates
Anthocyanins
cyanidin-3-O-beta-glucopyranoside