Presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection

Richard B M Schasfoort; Jos van Weperen; Margot van Amsterdam; Judicaël Parisot; Jan Hendriks; Michelle Koerselman; Marcel Karperien; Anouk Mentink; Martin Bennink; Hans Krabbe; Leon Wmm Terstappen; A H Leontine Mulder

doi:10.1016/j.bios.2021.113165

Presence and strength of binding of IgM, IgG and IgA antibodies against SARS-CoV-2 during CoViD-19 infection

Biosens Bioelectron. 2021 Jul 1:183:113165. doi: 10.1016/j.bios.2021.113165. Epub 2021 Mar 16.

Affiliations

¹ Department of Medical Cell BioPhysics, Faculty of Science and Technology, University of Twente, PO Box 217, 7500, AE, Enschede, the Netherlands. Electronic address: r.b.m.schasfoort@utwente.nl.
² Vysens BV, PO Box 50035, 7550, LA, Hengelo, the Netherlands.
³ Department of Medical Cell BioPhysics, Faculty of Science and Technology, University of Twente, PO Box 217, 7500, AE, Enschede, the Netherlands.
⁴ Carterra, 825 N. 300 W., Suite C309, Salt Lake City, UT, 84103, USA.
⁵ Department of Developmental BioEngineering, Faculty of Science and Technology, University of Twente, PO Box 217, 7500, AE, Enschede, the Netherlands.
⁶ NanoBio Research Group, Saxion University of Applied Sciences, PO Box 70000, 7500, KB, Enschede, the Netherlands.
⁷ Department of Clinical Chemistry and Laboratory Medicine, Medisch Spectrum Twente, PO Box 50000, 7500, KA, Enschede, the Netherlands; Department of Clinical Chemistry, Medlon, BV, 7512KZ, Enschede, the Netherlands.
⁸ Department of Clinical Chemistry, Medlon, BV, 7512KZ, Enschede, the Netherlands; Department of Clinical Chemistry and Laboratory Medicine, Ziekenhuis Groep Twente, PO BOX 7600, 7600, SZ, Almelo, the Netherlands.

Abstract

Surface Plasmon Resonance imaging (SPRi) was used to determine the presence and strength of binding of IgG, IgM and IgA against the Receptor Binding Domain (RBD) of SARS-CoV-2 in sera of 119 CoViD-19 patients. The SPRi assay measures the antibody isotype levels and the strength of binding to the RBD of ultimate 384 patient samples in one run. It turns out that during the course of the disease, the IgG levels and strength of binding increased while generally the IgM and IgA levels go down. Recovered patients all show high strength of binding of the IgG type to the RBD protein. The anti-RBD immunoglobulins SPRi assay provides additional insights in the immune status of patients recovering from CoViD-19 and this new method can furthermore be applied for the assessment of the quality of the immune reaction of healthy individuals to SARS-CoV-2 in vaccination programs.

Keywords: Affinity; Biosensor; SPR; Surface plasmon resonance imaging; off-rate.

MeSH terms

Antibodies, Viral
Biosensing Techniques*
COVID-19*
Humans
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M
SARS-CoV-2

Substances

Antibodies, Viral
Immunoglobulin A
Immunoglobulin G
Immunoglobulin M