Characterization of the complete chloroplast genome of a Chinese Endangered species Cymbidium mastersii

Qing Ling; Longjie Cheng; Zhilin Li; Yiran Zhao; Yuying Wang

doi:10.1080/23802359.2020.1851152

Characterization of the complete chloroplast genome of a Chinese Endangered species Cymbidium mastersii

Mitochondrial DNA B Resour. 2021 Mar 15;6(3):708-709. doi: 10.1080/23802359.2020.1851152.

Authors

Qing Ling^{1

2}, Longjie Cheng¹, Zhilin Li¹, Yiran Zhao¹, Yuying Wang¹

Affiliations

¹ College of Horticulture and Landscape, Yunnan Agricultural University, Kunming, China.
² Yunnan Vocational and Technical College of Agriculture, Kunming, China.

Abstract

Cymbidium mastersii is an endangered species belonging to the first ranking in protection category in China with important ornamental value and breeding value. This study used Illumina high-throughput sequencing technologies for C. mastersii of chloroplast genome sequencing. The genome features of C. mastersii and the phylogenetic relationships were reported and established. The complete chloroplast genome is 156,317 bp in length, consisting of a pair of inverse duplication regions 26,544 bp, a large single-copy region 85,360 bp and a small single-copy region 17,869 bp. The entire genome contains 73 mRNA genes, 30 tRNA genes and 4 rRNA genes. The phylogenetic tree of 23 Orchidaceae species revealed C. mastersii is more closely related to Cymbidium eburneum.

Keywords: Cymbidium mastersii; chloroplast genome; endangered species; phylogenetic analysis.

Grants and funding

This work was supported by the Yunnan Province Major Project of China [grant number 2018BB010], Yunnan Province Basic Research Project of China [grant number 2018FB070], Open Fund project of Yunnan Key Laboratory of Flower Breeding of China [grant number FKL-201602], Key Laboratory Project of Education Department of Yunnan Province of China and Innovation Team of Universities Project of Education Department of Yunnan Province of China.