Development of stabilized dual growth factor-loaded hyaluronate collagen dressing matrix

Jihyun Kim; Kyoung-Mi Lee; Seung Hwan Han; Eun Ae Ko; Dong Suk Yoon; Ik Kyu Park; Hang-Cheol Shin; Kwang Hwan Park; Jin Woo Lee

doi:10.1177/2041731421999750

Development of stabilized dual growth factor-loaded hyaluronate collagen dressing matrix

J Tissue Eng. 2021 Mar 16:12:2041731421999750. doi: 10.1177/2041731421999750. eCollection 2021 Jan-Dec.

Authors

Jihyun Kim^{1

2}, Kyoung-Mi Lee^{1

3}, Seung Hwan Han⁴, Eun Ae Ko¹, Dong Suk Yoon¹, Ik Kyu Park⁵, Hang-Cheol Shin⁶, Kwang Hwan Park¹, Jin Woo Lee^{1

2

3}

Affiliations

¹ Department of Orthopaedic Surgery, Yonsei University College of Medicine, Seoul, South Korea.
² Brain Korea 21 PLUS Project for Medical Sciences, Yonsei University College of Medicine, Seoul, South Korea.
³ Severance Biomedical Science Institute, Yonsei University College of Medicine, Seoul, South Korea.
⁴ Department of Orthopaedic Surgery, Yonsei University College of Medicine, Gangnam Severance Hospital, Seoul, South Korea.
⁵ R&D Center, Genewel Co., Ltd., Sungnam, South Korea.
⁶ School of Systems Biomedical Science, Soongsil University, Seoul, South Korea.

Abstract

Patients with diabetes experience impaired growth factor production such as epidermal growth factor (EGF) and basic fibroblast growth factor (bFGF), and they are reportedly involved in wound healing processes. Here, we report dual growth factor-loaded hyaluronate collagen dressing (Dual-HCD) matrix, using different ratios of the concentration of stabilized growth factors-stabilized-EGF (S-EGF) and stabilized-bFGF (S-bFGF). At first, the optimal concentration ratio of S-EGF to S-bFGF in the Dual-HCD matrix is determined to be 1:2 in type I diabetic mice. This Dual-HCD matrix does not cause cytotoxicity and can be used in vivo. The wound-healing effect of this matrix is confirmed in type II diabetic mice. Dual HCD enhances angiogenesis which promotes wound healing and thus, it shows a significantly greater synergistic effect than the HCD matrix loaded with a single growth factor. Overall, we conclude that the Dual-HCD matrix represents an effective therapeutic agent for impaired diabetic wound healing.

Keywords: Impaired wound healing; basic fibroblast growth factor; dual-hyaluronate collagen dressing matrix; epidermal growth factor; stabilized growth factor.