Comprehensive Two-Dimensional Gas Chromatography Mass Spectrometry-Based Metabolomics

Md Aminul Islam Prodhan; Craig McClain; Xiang Zhang

doi:10.1007/978-3-030-51652-9_4

Comprehensive Two-Dimensional Gas Chromatography Mass Spectrometry-Based Metabolomics

Adv Exp Med Biol. 2021:1280:57-67. doi: 10.1007/978-3-030-51652-9_4.

Authors

Md Aminul Islam Prodhan^{1

2

3

4}, Craig McClain^{2

3

5

6

7}, Xiang Zhang^{8

9

10

11

12}

Affiliations

¹ Department of Chemistry, University of Louisville, Louisville, KY, USA.
² University of Louisville Alcohol Research Center, University of Louisville, Louisville, KY, USA.
³ University of Louisville Hepatobiology & Toxicology Program, University of Louisville, Louisville, KY, USA.
⁴ Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, KY, USA.
⁵ Department of Pharmacology & Toxicology, University of Louisville, Louisville, KY, USA.
⁶ Department of Medicine, University of Louisville, Louisville, KY, USA.
⁷ Robley Rex Louisville VAMC, Louisville, KY, USA.
⁸ Department of Chemistry, University of Louisville, Louisville, KY, USA. xiang.zhang@louisville.edu.
⁹ University of Louisville Alcohol Research Center, University of Louisville, Louisville, KY, USA. xiang.zhang@louisville.edu.
¹⁰ University of Louisville Hepatobiology & Toxicology Program, University of Louisville, Louisville, KY, USA. xiang.zhang@louisville.edu.
¹¹ Center for Regulatory and Environmental Analytical Metabolomics, University of Louisville, Louisville, KY, USA. xiang.zhang@louisville.edu.
¹² Department of Pharmacology & Toxicology, University of Louisville, Louisville, KY, USA. xiang.zhang@louisville.edu.

PMID: 33791974
DOI: 10.1007/978-3-030-51652-9_4

Abstract

Compared to one-dimensional gas chromatography with mass spectrometry (GC-MS), GC × GC-MS provides significantly increased peak capacity, resolution, and sensitivity for analysis of complex biological samples. In the last decade, GC × GC-MS has been increasingly applied to the discovery of metabolite biomarkers and elucidation of metabolic mechanisms in human diseases. The recent development of coupling GC × GC with a high-resolution mass spectrometer further accelerates these metabolomic applications. In this chapter, we will briefly review the instrumentation, sample preparation, data analysis, and applications of GC × GC-MS-based metabolomic analysis.

Keywords: Column configuration; GC × GC-MS; Metabolomics; Sample preparation.

MeSH terms

Biomarkers
Gas Chromatography-Mass Spectrometry
Humans
Mass Spectrometry
Metabolomics*

Substances

Biomarkers