Development of novel bone-like nanocomposite coating of hydroxyapatite/collagen on titanium by modified electrophoretic deposition

J Biomed Mater Res A. 2021 Oct;109(10):1905-1911. doi: 10.1002/jbm.a.37182. Epub 2021 Mar 30.

Abstract

Electrophoretic deposition (EPD) is a simple, rapid, and inexpensive technique to accomplish uniform coatings with controlled thicknesses. The EPD using binders that do not require a thermal degreasing process, which also eliminates the polymer components of the composite, are required for coating polymer-ceramic composites. This study demonstrated the application of a modified EPD technique utilizing Mg2+ ions to coat a bone-like hydroxyapatite/collagen nanocomposite (HAp/Col) on a titanium (Ti) substrate. The coating thickness was successfully controlled by varying the applied voltage and/or the treatment time. The adhesive strength of the modified EPD coating, evaluated by the tape test, showed class 0 (coating was not peeled off) and drastically increased in comparison to that of the non-Mg2+ EPD coating, class 5 (coating was completely peeled off). The MG63 cells on the HAp/Col-coated Ti demonstrated similar proliferation to and superior alkaline phosphatase activity to that on the bare Ti. Thus, the HAp/Col-coated Ti is expected to facilitate the surrounding bone formation than the bare-Ti. The results of the study indicated the HAp/Col-coated Ti prepared by the modified EPD is effective for applications in novel instruments, such as, subperiosteal temporary anchorage devices, which strongly requires rapid osseointegration at the bone-implant surface.

Keywords: coating; electrophoretic deposition; hydroxyapatite/collagen; implant; orthodontic device.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adhesiveness
  • Alkaline Phosphatase / metabolism
  • Bone and Bones / drug effects
  • Bone and Bones / physiology*
  • Cell Line
  • Cell Survival / drug effects
  • Coated Materials, Biocompatible / pharmacology
  • Collagen / pharmacology*
  • DNA / metabolism
  • Durapatite / pharmacology*
  • Electrophoresis*
  • Humans
  • Nanocomposites / chemistry*
  • Nanocomposites / ultrastructure
  • Static Electricity
  • Surface Properties
  • Titanium / pharmacology*

Substances

  • Coated Materials, Biocompatible
  • Collagen
  • DNA
  • Durapatite
  • Titanium
  • Alkaline Phosphatase