A magnetic relaxation switch sensor for determination of 17β-estradiol in milk and eggs based on aptamer-functionalized Fe3 O4 @Au nanoparticles

Abstract

Background: A simple and rapid detection method for 17β-estradiol (E₂ ) in complex food matrix is greatly desirable. A magnetic relaxation switch (MRS) sensor for detecting E₂ based on the aptamer-functionalized gold-coated iron oxide (Fe₃ O₄ @Au) nanocomposite was designed in this study. Fe₃ O₄ @Au nanoparticles (NPs) played as a 'switch' between dispersed and aggregated states, while aptamer served as the recognition unit.

Results: According to the sensing effect of monocomponent relaxation time (T_2W ) for E₂ , the volume ratio of aptamers to Fe₃ O₄ @Au, the sodium chloride (NaCl) concentration, the concentration of Fe₃ O₄ @Au@Apt, and reaction time were optimized to be 4:1, 0.03 mol L^-1 , 4 μmol L^-1 and 15 min, respectively. For the analysis of food sample, the E₂ was quantified over a concentration range of 1 to 100 nmol L^-1 with a detection limit of 7.6 nmol L^-1 for milk samples, while a linearity range of 20 to 100 nmol L^-1 and a detection limit of 8.57 nmol L^-1 for egg samples.

Conclusion: These results exhibited that the MRS sensor could be a promising platform for the rapid detecting of E₂ in food sample. © 2021 Society of Chemical Industry.

Keywords: 17β-estradiol; Fe3O4@Au nanocomposites; aptamer; biosensor; magnetic relaxation switch sensor.