Dissection of Drought Tolerance in Upland Cotton Through Morpho-Physiological and Biochemical Traits at Seedling Stage

Zobia Zahid; Muhammad Kashif Riaz Khan; Amjad Hameed; Muhammad Akhtar; Allah Ditta; Hafiz Mumtaz Hassan; Ghulam Farid

doi:10.3389/fpls.2021.627107

Dissection of Drought Tolerance in Upland Cotton Through Morpho-Physiological and Biochemical Traits at Seedling Stage

Front Plant Sci. 2021 Mar 12:12:627107. doi: 10.3389/fpls.2021.627107. eCollection 2021.

Authors

Zobia Zahid^{1

2}, Muhammad Kashif Riaz Khan^{1

2}, Amjad Hameed^{1

2}, Muhammad Akhtar^{2

3}, Allah Ditta^{1

2}, Hafiz Mumtaz Hassan^{1

2}, Ghulam Farid³

Affiliations

¹ Plant Breeding and Genetics Division, Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.
² NIAB-C, Pakistan Institute of Engineering and Applied Science Nilore, Islamabad, Pakistan.
³ Soil and Environmental Sciences Division, Nuclear Institute for Agriculture and Biology, Faisalabad, Pakistan.

Abstract

Cotton is an important fiber and cash crop. Extreme water scarceness affects the growth, quality, and productivity of cotton. Water shortage has threatened the future scenario for cotton growers, so it is imperative to devise a solution to this problem. In this research, we have tried to machinate a solution for it. 23 genotypes have been screened out against drought tolerance at the seedling stage by evaluating the morphological, physiological, and biochemical traits in a triplicate completely randomized design plot experiment with two water regimes [50 and 100% field capacity]. Genotypic differences for all the morphological and physiological traits revealed highly significant differences except transpiration rate (TR). Moreover, the interaction between genotype and water regime (G × W) was highly significant for root length (RL, 5.163), shoot length (SL, 11.751), excised leaf water loss (ELWL, 0.041), and stomatal conductance (SC, 7.406). A positively strong correlation was found in TR with relative water content (RWC; 0.510) and SC (0.584) and RWC with photosynthesis (0.452) under drought conditions. A negative correlation was found in SC with SL (-0.428) and photosynthesis (-0.446). Traits like RL, SL, SC, photosynthesis, proline, catalase, and malondialdehyde were visible indicators, which can differentiate drought-tolerant genotypes from the susceptible ones. A wide range of diversity was found in all the morpho-physiological traits with the cumulative variance of four principal components (PCs) 83.09% and three PCs 73.41% under normal and water-stressed conditions, respectively, as per the principal component analysis. Hence, selection criteria can be established on the aforementioned traits for the development of drought-tolerant cultivars. Moreover, it was found that out of 23 experimental varieties, NIAB-135, NIAB-512, and CIM-554 could be used to devise breeding strategies for improving drought tolerance in cotton.

Keywords: Gossypium; catalase; root length; stomatal conductance; stress tolerance indices; water stress.