Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function

Lu Zhao; Wei Du; Dandan Zhao; Xueyan Ji; Yanfei Huang; Yong Pang; Kaijin Guo; Xiaoxing Yin

doi:10.3389/fphar.2021.626621

Catalpol Protects Against High Glucose-Induced Bone Loss by Regulating Osteoblast Function

Front Pharmacol. 2021 Mar 10:12:626621. doi: 10.3389/fphar.2021.626621. eCollection 2021.

Authors

Lu Zhao¹, Wei Du¹, Dandan Zhao², Xueyan Ji¹, Yanfei Huang¹, Yong Pang³, Kaijin Guo³, Xiaoxing Yin¹

Affiliations

¹ Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, China.
² Department of Emergency Medicine Center, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.
³ Department of Orthopedics, The Affiliated Hospital of Xuzhou Medical University, Xuzhou, China.

Abstract

Objective: The overall objective of this study was to investigate the effects of catalpol on bone remodeling of diabetic osteoporosis by regulating osteoblast differentiation and migration. Method: Using a murine model of diabetic osteoporosis, to detect the protective effects of catalpol on bone loss, architectural deterioration of trabecular bone and bone metabolism biomarkers were tested. A model of MC3T3-E1 cells was established by treatment with high glucose; the regulatory role of catalpol in the differentiation and migration was tested by Western blot, ALP staining, and Alizarin Red staining. Results: Catalpol treatment markedly ameliorated trabecular bone deterioration by reducing degenerative changes of the trabecular structure by improving the bone formation marker levels of ALP, osteopontin, type I collagen, and osteocalcin, as well as the level of OPG/RANKL. Catalpol enhanced cell motility and scattering following gap formation of MC3T3-E1 cells. Conclusion: The results indicated that catalpol exhibits a protective effect against diabetic osteoporosis by regulating the differentiation and migration of osteoblast.

Keywords: catalpol; diabetic osteoporosis (DOP); differentiation; migration; osteoblast.