A copper nanoparticle-based electrochemical immunosensor for carbaryl detection

Elena V Dorozhko; Anna S Gashevskay; Elena I Korotkova; Jiri Barek; Vlastimil Vyskocil; Sergei A Eremin; Evgeny V Galunin; Muhammad Saqib

doi:10.1016/j.talanta.2021.122174

A copper nanoparticle-based electrochemical immunosensor for carbaryl detection

Talanta. 2021 Jun 1:228:122174. doi: 10.1016/j.talanta.2021.122174. Epub 2021 Feb 4.

Authors

Elena V Dorozhko¹, Anna S Gashevskay², Elena I Korotkova³, Jiri Barek⁴, Vlastimil Vyskocil⁵, Sergei A Eremin⁶, Evgeny V Galunin⁷, Muhammad Saqib⁸

Affiliations

¹ School of Earth Sciences and Engineering, Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia. Electronic address: elena-dorozhko@yandex.ru.
² School of Earth Sciences and Engineering, Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia. Electronic address: www.anyutka.kz@mail.ru.
³ School of Earth Sciences and Engineering, Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia. Electronic address: eikor@mail.ru.
⁴ School of Earth Sciences and Engineering, Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia; Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 12843, Prague 2, Czech Republic. Electronic address: barek@natur.cuni.cz.
⁵ Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 12843, Prague 2, Czech Republic. Electronic address: vlastimil.vyskocil@natur.cuni.cz.
⁶ M. Lomonosov Moscow State University, 1 Leninskie Gory, 119991, Moscow, Russia. Electronic address: eremin_sergei@hotmail.com.
⁷ Research School of Chemistry and Applied Biomedical Sciences, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia. Electronic address: evgeny.galunin@gmail.com.
⁸ School of Earth Sciences and Engineering, Department of Chemical Engineering, National Research Tomsk Polytechnic University, Lenin Avenue 30, 634050, Tomsk, Russia; Charles University, Faculty of Science, Department of Analytical Chemistry, UNESCO Laboratory of Environmental Electrochemistry, Albertov 6, 12843, Prague 2, Czech Republic. Electronic address: ms.engr9@gmail.com.

PMID: 33773707
DOI: 10.1016/j.talanta.2021.122174

Abstract

A hapten-protein conjugate with copper nanoparticles (Hap-Car-BSA@CuNPs) was first synthesized in the present work for the determination of carbaryl. The copper nanoparticles (CuNPs) of the conjugate were used as electrochemical labels in the direct solid-phase competitive determination of carbaryl residues in flour from different crops. The signal was read by linear sweep anodic stripping voltammetry (LSASV) of copper (through the electrochemical stripping of accumulated elemental copper) on a gold-graphite electrode (GGE). To form a recognition receptor layer of monoclonal antibodies against the carbaryl on the surface of the GGE, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (EDC) and 1-hydroxy-2,5-pyrrolidinedione (NHS) were used as the best covalent cross-linkers. The concentrations of the antibodies and the Hap-Car-BSA@CuNPs conjugate were optimized for carbaryl detection by the electrochemical immunosensor. The electrochemical immunosensor can be used for highly sensitive determination of carbaryl residues in flour samples in the concentration range 0.8-32.3 μg·kg^-1, with a limit of detection 0.08 μg·kg^-1. The present work paves the path for a novel method for monitoring carbaryl in other food products, drinks, and soil samples.

Keywords: Antibodies; Carbaryl; Copper nanoparticles; Electrochemical immunosensor; Gold-graphite electrode; Linear sweep anodic stripping voltammetry.

MeSH terms

Biosensing Techniques*
Carbaryl
Copper
Electrochemical Techniques
Gold
Immunoassay
Limit of Detection
Metal Nanoparticles*
Nanoparticles*

Substances

Gold
Copper
Carbaryl