Complete nucleotide sequence of a novel partitivirus from Brassica campestris L. ssp. chinensis

Arch Virol. 2021 Jun;166(6):1775-1778. doi: 10.1007/s00705-021-05041-x. Epub 2021 Mar 26.

Abstract

In the present work, we report the discovery and complete genome sequence of a novel partitivirus identified from Brassica campestris L. ssp. chinensis, which we have named "Brassica campestris chinensis cryptic virus 1" (BCCV1). Next-generation sequencing (NGS) combined with adapter-ligation-mediated amplification allowed assembly of the full-length genome sequence of BCCV1. The genome of BCCV1 contains two dsRNA segments, dsRNA1 (1595 bp) and dsRNA2 (1591 bp), which encode a conserved RNA-dependent RNA polymerase (RdRp) and a putative capsid protein (CP), respectively. Homology searches and phylogenetic analysis of the 479-aa RdRp and 438-aa CP showed that BCCV1 is a new member of the genus Deltapartitivirus, family Partitiviridae. This is the first report of the identification of a member of the family Partitiviridae in Brassica campestris L. ssp. chinensis.

MeSH terms

  • Base Sequence
  • Brassica / virology*
  • Phylogeny
  • Plant Diseases / virology*
  • Plant Viruses / genetics*
  • RNA / genetics*

Substances

  • RNA