A carboxymethyl lentinan layer by layer self-assembly system as a promising drug chemotherapeutic platform

Duanhua Cai; Linrong Shi; Ruimin Long; Gang Ren; Shibin Wang; Yuangang Liu

doi:10.1016/j.carbpol.2021.117847

A carboxymethyl lentinan layer by layer self-assembly system as a promising drug chemotherapeutic platform

Carbohydr Polym. 2021 Jun 1:261:117847. doi: 10.1016/j.carbpol.2021.117847. Epub 2021 Feb 26.

Authors

Duanhua Cai¹, Linrong Shi¹, Ruimin Long², Gang Ren³, Shibin Wang⁴, Yuangang Liu⁵

Affiliations

¹ College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China.
² College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China. Electronic address: simplever@126.com.
³ College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, China.
⁴ Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, 361021, China; Fujian Provincial Key Laboratory of Biochemical Technology, Xiamen, 361021, China; College of Materials Science and Engineering, Huaqiao University, Xiamen, 361021, China.
⁵ College of Chemical Engineering, Huaqiao University, Xiamen, 361021, China; Institute of Pharmaceutical Engineering, Huaqiao University, Xiamen, 361021, China; Fujian Provincial Key Laboratory of Biochemical Technology, Xiamen, 361021, China. Electronic address: ygliu@hqu.edu.cn.

PMID: 33766343
DOI: 10.1016/j.carbpol.2021.117847

Abstract

Surface functionalization of mesoporous silica nanoparticles (MSNs) has been proposed as an efficient strategy for enhancing the biocompatibility and efficiency of an MSN-based carrier platform. Herein, natural polyelectrolyte multilayers composed of poly-l-ornithine (PLO) and carboxymethyl lentinan (LC) were coated on the surface of MSNs through a layer-by-layer (LbL) self-assembly technique, and were characterized by ζ-potential, FTIR, ¹³C NMR, SEM, TEM, XRD, and TG. The prepared carrier presented alternating positive and negative potentials when coated with the polyelectrolytes, and the surface of MSN-PLO/LC was rougher compared to the naked MSNs. The biocompatibility tests, including cytocompatibility, hemocompatibility, and histocompatibility, showed that MSNs biocompatibility could be improved by modifying LC. A high loading and sustained release drug delivery system was constructed after loading doxorubicin (DOX) into the prepared MSN-PLO/LC, which exhibited significant anti-proliferative efficiency in human cervical cancer cell lines (Hela). Therefore, the PLO/LC LbL NPs (layer-by-layer self-assembled nanoparticles coated with PLO/LC layers) based on MSNs, which is easily prepared by electrostatic interactions, can be considered a promising drug chemotherapeutic platform and delivery technique for future human cervical cancer therapy.

Keywords: Anti-tumour; Layer by layer self-assembly; Lentinan; Mesoporous silica; PLO.

MeSH terms

Animals
Antineoplastic Agents / administration & dosage*
Antineoplastic Agents / pharmacokinetics
Cells, Cultured
Drug Carriers* / chemical synthesis
Drug Carriers* / chemistry
Drug Carriers* / therapeutic use
Drug Compounding
Drug Delivery Systems
Drug Liberation
Female
HeLa Cells
Humans
Lentinan* / analogs & derivatives
Lentinan* / chemical synthesis
Lentinan* / chemistry
Lentinan* / therapeutic use
Male
Materials Testing
Mice
Nanoparticles / chemistry
Nanoparticles / therapeutic use
Polymerization
Polymers / chemical synthesis
Polymers / chemistry
Polymers / therapeutic use
Porosity
Rabbits
Silicon Dioxide / chemistry
Xenograft Model Antitumor Assays

Substances

Antineoplastic Agents
Drug Carriers
Polymers
Lentinan
Silicon Dioxide