Mapping chromosomal regions associated with anther indehiscence with exerted stigmas in CRI-48 and Jasmine 85 cross of rice (Oryza sativa L)

Samuel Oppong Abebrese; Nana Kofi Abaka Amoah; Paul Kofi Ayirebi Dartey; Isaac Kofi Bimpong; Richard Akromah; Vernon Edward Gracen; Samuel Kwame Offei; Eric Yirenkyi Danquah

doi:10.1016/j.heliyon.2021.e06483

Mapping chromosomal regions associated with anther indehiscence with exerted stigmas in CRI-48 and Jasmine 85 cross of rice (Oryza sativa L)

Heliyon. 2021 Mar 13;7(3):e06483. doi: 10.1016/j.heliyon.2021.e06483. eCollection 2021 Mar.

Authors

Samuel Oppong Abebrese¹, Nana Kofi Abaka Amoah², Paul Kofi Ayirebi Dartey³, Isaac Kofi Bimpong², Richard Akromah⁴, Vernon Edward Gracen⁵, Samuel Kwame Offei⁵, Eric Yirenkyi Danquah⁵

Affiliations

¹ CSIR-Savanna Agricultural Research Institute, P.O. Box TL 52, Tamale, Ghana.
² Africa Rice Centre, Headquarters, M'bé Research Station. 01 B.P 2551, Bouaké o1, Cote d'Ivoire.
³ CSIR-Crops Research Institute, P. O. Box 3785, Fumesua, Ghana.
⁴ Department of Crop and Soil Sciences, KNUST, Kumasi, Ghana.
⁵ West Africa Centre for Crop Improvement, University of Ghana, Legon, Ghana.

Abstract

Anther indehiscence in certain wide crosses combines male sterility with stigma exertion, a phenomenon that is desirable for hybrid rice seed production. This study sought to identify chromosomal region(s) that combine anther indehiscence with exerted stigmas. A mapping population consisting of 189 BC₁F₁ plants was derived from a cross between CRI-48 and Jasmine 85 and backcrossing the resulting F₁ to Jasmine 85. Contrary to the three complementary genes mode of inheritance reported earlier, a single locus (AI6-1) was mapped on chromosome 6 at 27.4 cM for anther indehiscence with exerted stigmas through a mixed model-based composite interval mapping (MCIM). This locus was flanked by two single nucleotide polymorphism (SNP) markers, K_ID6002884 and K_ID6003341 within a range of 23.1-28.9 cM. The allele at the locus was contributed by the CRI-48 parent which has Oryza glaberrima ancestry. This locus is suggested to control anther indehiscence and stigma exertion through pleiotropic gene action or cluster of genes.

Keywords: Genetic mapping; Hybrid rice seed production; Outcrossing; Pleiotropic gene action; SNP markers.