Characterization and Functional Test of Canine Probiotics

Hyun-Jun Jang; Seungwoo Son; Jung-Ae Kim; Min Young Jung; Yeon-Jae Choi; Dae-Hyuk Kim; Hak Kyo Lee; Donghyun Shin; Yangseon Kim

doi:10.3389/fmicb.2021.625562

Characterization and Functional Test of Canine Probiotics

Front Microbiol. 2021 Mar 8:12:625562. doi: 10.3389/fmicb.2021.625562. eCollection 2021.

Authors

Hyun-Jun Jang¹, Seungwoo Son^{2

3}, Jung-Ae Kim^{1

4}, Min Young Jung¹, Yeon-Jae Choi¹, Dae-Hyuk Kim^{1

4

5}, Hak Kyo Lee^{2

3}, Donghyun Shin^{2

3}, Yangseon Kim¹

Affiliations

¹ Department of Research and Development, Center for Industrialization of Agricultural and Livestock Microorganisms, Jeongeup-si, South Korea.
² Department of Agricultural Convergence Technology, Jeonbuk National University, Jeonju-si, South Korea.
³ The Animal Molecular Genetics & Breeding Center, Jeonbuk National University, Jeonju-si, South Korea.
⁴ Department of Bioactive Material Sciences, Jeonbuk National University, Jeonju-si, South Korea.
⁵ Department of Molecular Biology, Institute for Molecular Biology and Genetics, Jeonbuk National University, Jeonju-si, South Korea.

Abstract

Probiotics can modulate the composition of gut microbiota and benefit the host animal health in multiple ways. Lactic acid bacteria (LAB), mainly Lactobacillus and Bifidobacterium species, are well-known microbes with probiotic potential. In the present study, 88 microbial strains were isolated from canine feces and annotated. Among these, the four strains CACC517, 537, 558, and 566 were tested for probiotic characteristics, and their beneficial effects on hosts were evaluated both in vitro and in vivo; these strains exhibited antibiosis, antibiotic activity, acid and bile tolerance, and relative cell adhesion to the HT-29 monolayer cell line. Byproducts of these strains increased the viability and decreased oxidative stress in mouse and dog cell lines (RAW264.7 and DH82, respectively). Subsequently, when the probiotics were applied to the clinical trial, changes in microbial composition and relative abundance of bacterial strains were clearly observed in the experimental animals. Experimental groups before and after the application were obviously separated from PCA analysis of clinical results. Conclusively, these results could provide comprehensive understanding of the effects of probiotic strains (CACC517, 537, 558, and 566) and their industrial applications.

Keywords: Bifidobacterium; Lactobacillus; canine; feed additives; probiotics.