This is one of the first studies carried out over three climatic seasons on the determination of ibuprofen and diclofenac, in the main rivers of Colombia and striped catfish Pseudoplatystoma magdaleniatum. Determination of water concentrations was carried out using SPE extraction, while for the analysis of the muscular tissue, the extraction was carried out by QuEChERS. For both matrices, quantification was done by UHPLC-MS/MS. No levels of ibuprofen or diclofenac concentrations were found in the muscle tissue of Pseudoplatystoma magdaleniatum, in any season or sampling site, during the 2 years of sampling. In some sampling sites, concentrations of up to 75 µg/L of diclofenac were detected, corresponding to the sampling carried out in the dry season, being the highest reported so far in surface waters, possibly generated by large concentrations of population or agricultural activities. On the other hand, for ibuprofen, no concentrations above the limit of quantification (0.50 µg/L) were found in the waters of the Cauca and Magdalena rivers, for any season and sampling site.
Keywords: Contamination; Fish; Non-steroidal anti-inflammatory drugs; QuEChERS; Surface water; UHPLC-MS/MS.