Establishing monoculture of native broadleaved tree species and mixed coniferous broadleaved plantations is the tendency for forest management in subtropical China. The variations of structure and function of soil bacterial community in monoculture and mixed tree plantations are still not clear. We examined soil bacterial community structure and function under different soil layers (0-20, 20-40 and 40-60 cm) in three planted forests, including broadleaved Castanopsis hystrix, coniferous Pinus massoniana and their mixed plantation, in south subtropical China, using 16S rRNA gene high-throughput sequencing and PICRUSt prediction. The results showed that soil bacterial community structure of mixed plantation and P. massoniana plantation were similar but being significant different from that in C. hystrix plantation. The diversity, biological pathways metabolic function, and nitrogen cycling function of soil bacterial community in C. hystrix plantation were lower than those in P. massoniana plantation and mixed plantation. Soil total nitrogen, nitrate nitrogen and C/N were the main factors driving the variations of soil bacterial community structure and function among different forest types. Our results suggested that the mixed plantation of C. hystrix and P. massoniana is better than C. hystrix plantation in this area in terms of soil bacterial community structure and function.
营建乡土阔叶树种人工纯林和针阔混交林是我国亚热带地区森林经营的发展趋势,但对人工纯林和针阔混交林土壤细菌群落结构及功能知之甚少。本研究以南亚热带乡土针叶树种马尾松、阔叶树种红椎人工纯林及二者的混交林为对象,运用细菌16S rRNA基因高通量测序技术和PICRUSt基因功能预测,分析了3种人工林不同土层(0～20、20～40和40～60 cm)土壤细菌群落的结构与功能。结果表明: 混交林和马尾松林土壤细菌群落结构相似,但与红椎林差异显著,红椎林土壤细菌群落多样性、生物通路代谢功能和氮循环功能低于马尾松林和混交林;土壤全氮、硝态氮和C/N是导致红椎林与马尾松林和混交林土壤细菌群落结构及功能差异的主要土壤理化因子。就土壤细菌群落结构与功能而言,在该地区营造红椎和马尾松针阔混交林要优于红椎纯林。.
Keywords: PICRUSt prediction; high-throughput sequencing; nitrogen cycling functional genes; plantation; soil bacterial community.