Strong and nonspecific synergistic antibacterial/antibiofilm impact of nano-silver biosynthesized and decorated with active ingredients of Oscimum basilicum L

Lakshmanan Muthulakshmi; T Vijayakumar; P Selvam; J Annaraj; Shivendu Ranjan; Nandita Dasgupta

doi:10.1007/s13205-021-02687-x

Strong and nonspecific synergistic antibacterial/antibiofilm impact of nano-silver biosynthesized and decorated with active ingredients of Oscimum basilicum L

3 Biotech. 2021 Apr;11(4):153. doi: 10.1007/s13205-021-02687-x. Epub 2021 Mar 3.

Authors

Lakshmanan Muthulakshmi^{1

2}, T Vijayakumar², P Selvam³, J Annaraj¹, Shivendu Ranjan⁴, Nandita Dasgupta⁴

Affiliations

¹ Department of Biotechnology, Kalasalingam Academy of Research and Education, Krishnankoil, 626 126 India.
² Department of Materials Science, Madurai Kamaraj University, Palkalai Nagar, Madurai, Tamil Nadu 625 021 India.
³ International Research Center, Kalasalingam Academy of Research and Education, Krishnankoil, 626 126 India.
⁴ Institute for Intelligent Systems, Faculty of Engineering and the Built Environment, University of Johannesburg, Auckland Park, P. O. Box 524, Johannesburg, 2006 South Africa.

Abstract

In this study, Ocimum basilicum (a proven broad spectrum medicinal plant for broad-spectrum pharmacological activities) leaf extract was used as conjugates for the fabrication of silver nanoparticles (AgNP). Color change of the reaction mixture and UV-Visible spectrophotometry indicated the fabrication of silver nanoparticles, further X-ray diffraction (XRD) crystallography, scanning electron microscopy (SEM), transmission electron microscopic images (TEM), and Selected area electron diffraction (SAED) confirms the purity, monodispersity, and morphology including size (22.4 nm) and conjugated functional group of Ocimum basilicum. The conjugation of functional OH, N-O, and C=O groups was confirmed by Fourier-transform infrared spectroscopy (FT-IR). The engineered AgNP have shown significantly efficient antibacterial and antibiofilm activities (92.7% biofilm inhibition) on diverse clinical strains and thus showed its potential for use in clinical applications.

Keywords: Antibacterial; Antibiofilm; Clinical microbiology; Ocimum basillicum; Pathogens; Silver nanoparticles.