A novel Gram-negative bacterial strain BT320T was isolated from soil collected in Uijeongbu city, Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BT320T belong to a distinct lineage within the genus Flavisolibacter (family Chitinophagaceae, order Chitinophagales, class Chitinophagia). The strain BT320T was closely related to Flavisolibacter galbus 17J28-26T (97.6% 16S rRNA gene similarity), Flavisolibacter nicotianae X7XT (96.7%), Flavisolibacter ginsengiterrae Gsoil 492T (96.2%), and Flavisolibacter ginsengisoli Gsoil 643 T (96.1%). The genome size of strain BT320T was 5,664,094 bp. Bacterial growth was observed at 10-37 °C (optimum 25 °C) and pH 6.0-8.0 (optimum pH 7.0) on R2A agar. The major cellular fatty acids of strain BT320T were iso-C15:0, summed feature 3 (C16:1 ω6c/C16:1 ω7c), and summed feature 1 (iso-C15:1 H/C13:0 3OH). Its predominant respiratory quinone was MK-7. The major polar lipid of strain BT320T was identified to be phosphatidylethanolamine (PE). Based on the biochemical, chemotaxonomic, and phylogenetic analysis, strain BT320T can be suggested as a novel bacterial species within the genus Flavisolibacter and the proposed name is Flavisolibacter longurius. The type strain of Flavisolibacter longurius is BT320T (= KCTC 72422T = NBRC 114375T).
Keywords: Chitinophagaceae; Flavisolibacter; Novel species; Taxonomy.
© 2021. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.