Analysis of Mono-ADP-Ribosylation Levels in Human Colorectal Cancer

Chuan-Ling Wang; Yi Tang; Ming Li; Ming Xiao; Qing-Shu Li; Lian Yang; Xian Li; Ling Yin; Ya-Lan Wang

doi:10.2147/CMAR.S303064

Analysis of Mono-ADP-Ribosylation Levels in Human Colorectal Cancer

Cancer Manag Res. 2021 Mar 12:13:2401-2409. doi: 10.2147/CMAR.S303064. eCollection 2021.

Authors

Chuan-Ling Wang¹, Yi Tang¹, Ming Li¹, Ming Xiao¹, Qing-Shu Li¹, Lian Yang¹, Xian Li¹, Ling Yin¹, Ya-Lan Wang¹

Affiliation

¹ Department of Pathology, Molecular Medicine and Cancer Research Center, Chongqing Medical University, Chongqing, 400016, People's Republic of China.

Abstract

Background: Colorectal cancer remains a major public health problem with high morbidity and mortality rates. In the search for the mechanisms of colorectal cancer occurrence and development, increasing attention has been focused on epigenetics. The overall level of Mono-ADP-ribosylation, an epigenetic, has not been investigated now. The aim of our study was to analysis of the overall level of mono-ADP-ribosylation in colorectal cancer.

Methods: Immunohistochemistry was used to investigate the level of mono-ADP-ribosylation in colorectal cancer and normal colorectal adjacent tissue from 64 CRC patients. The data of patient demographic, clinical and pathological characteristics were acquired and analyzed.

Results: Mono-ADP-ribosylation was present in both colorectal adenocarcinoma and normal colorectal tissue. The overall level of mono-ADP-ribosylation in colorectal cancer was significantly higher than that in normal colorectal adjacent tissue. In the nucleus, the majority of samples in the high-level group were colorectal adenocarcinoma (55/64), but the opposite was true for normal colorectal tissues (7/32). In particular, increases in the level of mono-ADP-ribosylation in the cytoplasm of colorectal cancer cells was associated with a greater invasion depth of the tumor.

Conclusion: The increased level of mono-ADP-ribosylation in colorectal cancer enhances tumor invasion, which suggests that mono-ADP-ribosylation is involved in the development of colorectal cancer and may become a new direction to solve the problem of colorectal cancer.

Keywords: colorectal cancer; immunohistochemistry; mono-ADP-ribosylation.

Grants and funding

The research was supported by the Innovation Project of Graduate Student in Chongqing (grant no. CYB17100); the Science and Technology Research Foundation of Chongqing Municipal Education Commission (KJQN 201800435); the Science and Technology Research Foundation of Chongqing Municipal Education Commission (KJQN 201900403); the Innovation Project of Graduate Student in Chongqing (CYB19160); and the Foundation and Cutting Edge Research Project from the Science and Technology Commission of Chongqing (cstc2016jcyjA0233).