Metabolic and physiological alterations indicate that the tropical broadleaf tree Eugenia uniflora L. is sensitive to ozone

Abstract

Eugenia uniflora L. is an important fruit tree native to tropical South America that adapts to different habitats, thanks to its metabolic diversity and ability to adjust the leaf antioxidant metabolism. We hypothesized that this metabolic diversity would also enable E. uniflora to avoid oxidative damage and tolerate the enhanced ozone (O3) concentrations that have been registered in the (sub)tropics. We investigated whether carbohydrates, polyphenols and antioxidants are altered and markers of oxidative damage (ROS accumulation, alterations in leaf gas exchange, growth and biomass production) are detected in plants exposed to two levels of O₃ (ambient air and twice elevated ozone level in a O₃-FACE system for 75 days). Phytotoxic O₃ dose above a threshold of 0 nmol m^-2 s^-1 (POD0) and accumulated exposure above 40 ppb (AOT40) were 3.6 mmol m^-2 and 14.898 ppb h at ambient, and 4.7 mmol m^-2 and 43.881 ppb h at elevated O₃. Twenty-seven primary metabolites and 16 phenolic compounds were detected in the leaves. Contrary to the proposed hypothesis that tropical broadleaf trees are relatively O₃ tolerant, we concluded that E. uniflora plants are sensitive to elevated O₃ concentrations. Experimental POD0 values were lower than the critical levels for visible foliar O₃, because of low stomatal conductance. In spite of this low stomatal O₃ uptake, we found classic O₃ injury, e.g. reduction in carbohydrates and fatty acids concentrations; non-significant changes in the polyphenol profile; inefficient antioxidant responses; increased contents of ROS and indicators of lipid peroxidation; reductions in stomatal conductance, net photosynthesis, root/shoot ratio and height growth. However, we also found some compensation mechanisms, e.g. increased leaf concentration of polyols for protecting the membranes, and increased leaf number for compensating the decline of photosynthetic rate. These results help filling the knowledge gap about tropical tree responses to O₃.

Keywords: Carbohydrates; Flavonoids; Growth and biomass; Photosynthesis; Reactive oxygen species; Tropical species.