Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions

Yanling Jin; Wei Liu; Fengxia Wang; Min Wang; Kai Xu; Aijun Yang; Chenyu Wang; Lihan Zhang; Fangfang Zhang; Min Li

doi:10.1080/19336918.2021.1898709

Tissue factor potentiates adherence of breast cancer cells to human umbilical vein endothelial cells under static and flow conditions

Cell Adh Migr. 2021 Dec;15(1):74-83. doi: 10.1080/19336918.2021.1898709.

Authors

Yanling Jin¹, Wei Liu¹, Fengxia Wang¹, Min Wang¹, Kai Xu², Aijun Yang¹, Chenyu Wang¹, Lihan Zhang³, Fangfang Zhang¹, Min Li^{1

4}

Affiliations

¹ Institute of Pathology, School of Basic Medical Sciences, Lanzhou University, Lanzhou, China.
² First Affiliated Clinical Hospital, Lanzhou University, Lanzhou, Gansu, China.
³ Department of Integrated Traditional Chinese and Western Medicine, The Affiliated Cancer Hospital of Zhengzhou University, Zhengzhou, Henan, China.
⁴ Gansu Provincial Key Laboratory of Preclinical Study for New Drug Development, Lanzhou University, Lanzhou, China.

Abstract

Tissue factor (TF) has been extensively studied for tumor metastasis, but its role in mediating cancer cell adhesion to vasculature remains unknown. This study aimed to measure the ability of TF to mediate the adhesion of breast cancer cells to human umbilical vein endothelial cells (HUVECs). MDA-MB-231 cells expressed the highest TF level and adhered more to HUVECs under static and flow conditions, a neutralizing TF antibody abolished the enhanced adhesion of MDA-MB-231 cells to HUVECs. Recombinant human soluble TF (rTF) bonded β1integrin on HUVECs surfaces, β1 or α3integrin antibody combined with TF antibody abolished more cell-cell adhesion. These data suggested that TF mediated adhesion of breast cancer cells to endothelial cells may rely on β1integrin on HUVECs surfaces.

Keywords: Tissue factor; adhesion; breast cancer; endothelium; β1 integrin.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antibodies, Monoclonal / pharmacology
Breast Neoplasms / metabolism*
Breast Neoplasms / pathology
Cell Adhesion / physiology*
Cells, Cultured
Endothelium, Vascular / metabolism*
Female
Human Umbilical Vein Endothelial Cells
Humans
Integrin beta1 / pharmacology
Integrin beta3 / pharmacology
Thromboplastin / metabolism*

Substances

Antibodies, Monoclonal
Integrin beta1
Integrin beta3
Thromboplastin

Grants and funding

This study was supported by the National Natural Science Foundation of China 81871919 and 81672399 (M.L), the Fundamental Research Funds for the Central Universities lzujbky-2016-62 (W.L) and lzujbky-2019-it12 (YL.J)