The Differentiation of Human Induced Pluripotent Stem Cells into Podocytes In Vitro

Andrea F Wise; Sheetal Saini; Sharon D Ricardo

doi:10.1007/7651_2021_370

The Differentiation of Human Induced Pluripotent Stem Cells into Podocytes In Vitro

Methods Mol Biol. 2022:2454:317-325. doi: 10.1007/7651_2021_370.

Authors

Andrea F Wise¹, Sheetal Saini¹, Sharon D Ricardo²

Affiliations

¹ Department of Pharmacology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia.
² Department of Pharmacology, Biomedicine Discovery Institute, Monash University, Clayton, VIC, Australia. sharon.ricardo@monash.edu.

PMID: 33733390
DOI: 10.1007/7651_2021_370

Abstract

Induced pluripotent stem cells (iPSCs) hold enormous potential in the field of regenerative medicine due to their pluripotent properties, where they can give rise to all cell types in the body. Here we describe a detailed 20-day culture and differentiation protocol to generate iPSC-derived podocytes grown as a monolayer. These iPSC-derived podocytes appear arborised by morphology and express podocyte-specific markers. Also described is a detailed immunofluorescence staining protocol to confirm successful differentiation using the podocyte-specific markers, Wilms' tumor protein (WT1) and podocin.

Keywords: Cell culture; Directed differentiation; Immunofluorescence; Podocin; Podocyte; WT-1; iPSC.

MeSH terms

Cell Differentiation
Humans
Induced Pluripotent Stem Cells*
Podocytes* / metabolism