Evaluation of Toxicity with Brine Shrimp Assay

Christina N Banti; Sotiris K Hadjikakou

doi:10.21769/BioProtoc.3895

Evaluation of Toxicity with Brine Shrimp Assay

Bio Protoc. 2021 Jan 20;11(2):e3895. doi: 10.21769/BioProtoc.3895.

Authors

Christina N Banti¹, Sotiris K Hadjikakou¹

Affiliation

¹ Section of Inorganic and Analytical Chemistry, Department of Chemistry, University of Ioannina, 45110 Ioannina, Greece.

Abstract

The in vivo toxicity of new metallodrugs either as Small Bioactive Molecules (SBAMs) or Conjugates of Metals with Drugs (CoMeDs) or their hydrogels such as with hydroxyethyl-methacrylate (HEMA) (pHEMA@SBAMs or pHEMA@CoMeDs) are evaluated by the brine shrimp assay. Thus individuals of Artemia salina larvae are incubated in saline solutions with SBAMs, CoMeDs, pHEMA@SBAMs or pHEMA@CoMeDs or without for 24 h. The toxicity is then determined in terms of the mortality rate of brine shrimp larvae. Brine shrimp assay is a low cost, safe, no required feeding during the assay, while it requiring only a small amount of the tested agent.

Keywords: Artemia salina; Bioinorganic Chemistry; Larvae mortality of brine shrimp; Toxicity.