Identification of carbapenems resistant genes on biofilm forming K. pneumoniae from urinary tract infection

Govindan Nadar Rajivgandhi; Naiyf S Alharbi; Shine Kadaikunnan; Jamal M Khaled; Chelliah Chenthis Kanisha; Govindan Ramachandran; Natesan Manoharan; Khalid F Alanzi

doi:10.1016/j.sjbs.2020.12.016

Identification of carbapenems resistant genes on biofilm forming K. pneumoniae from urinary tract infection

Saudi J Biol Sci. 2021 Mar;28(3):1750-1756. doi: 10.1016/j.sjbs.2020.12.016. Epub 2020 Dec 29.

Authors

Govindan Nadar Rajivgandhi¹, Naiyf S Alharbi², Shine Kadaikunnan², Jamal M Khaled², Chelliah Chenthis Kanisha³, Govindan Ramachandran¹, Natesan Manoharan¹, Khalid F Alanzi²

Affiliations

¹ Department of Marine Science, Bharathidasan University, Tiruchirappalli 620024, Tamil Nadu, India.
² Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.
³ Noorul Islam Centre for Higher Education, Kumaracoil, Kanyakumari, Tamil Nadu 629180, India.

Abstract

The multi-drug resistant effect of the Gram negative bacteria K. pneumoniae was identified by disc diffusion method using specific UTI panel discs of Kleb 1 HX077 and Kleb 2 HX090 HEXA. Among the multi-drug resistant bacteria, the carbapenem resistant (CR) effect of the K. pneumoniae was screened by specific carbapenem detection antibiotics of HEXA HX066 and HX0103 HEXA by disc diffusion method. In addition, the effective antibiotics were further performed against K. pneumoniae by minimum inhibition concentration method. Further, the carbapenemase genes of VIM 1 and IMP 1 were detected from the isolated strains by multiplex PCR method. Furthermore, the biofilm forming ability of selected carbapenem resistant K. pneumoniae was initially identified by tissue culture plate method and confirmed by exopolysaccharide arrest ability of congo red agar assay. Finally, our result was proved that the identified K. pneumoniae is carbapenemase producing strain, and its virulence was extended with strong biofilm formation.

Keywords: Antibiotics; Biofilm formation; Gram negative bacteria; Minimum inhibition concentration; Multi-drug resistant bacteria; Urinary tract infections.