Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors

Guangli Zhu; Yuting Zhang; Jianjun Dong; Yilin Liu; Fang Zhao; Ting Li; Zhanrong Shi; Yanping Zhang; Na Song; Wenyue Song; Yuzhen Lv

doi:10.2147/DMSO.S289923

Association Between Body Mass Index and Male Sperm Apoptosis and Apoptosis-Related Factors

Diabetes Metab Syndr Obes. 2021 Mar 9:14:1043-1051. doi: 10.2147/DMSO.S289923. eCollection 2021.

Authors

Guangli Zhu¹, Yuting Zhang¹, Jianjun Dong¹, Yilin Liu¹, Fang Zhao¹, Ting Li¹, Zhanrong Shi¹, Yanping Zhang¹, Na Song¹, Wenyue Song¹, Yuzhen Lv¹

Affiliation

¹ Reproductive Medicine Center, Jiaozuo Women and Children's Hospital, Jiaozuo, Henan Province, People's Republic of China.

Abstract

Background: Although obesity may affect reproductive functions, the molecular mechanisms of apoptosis-related biomarkers remain uncertain.

Objective: To examine the effects of body mass index on sperm quality and apoptosis-related factors in seminal plasma of men.

Methods: Data for 54 subfertile men were collected at our reproductive medical center. The men were divided into normal weight, overweight, and obese groups based on their body mass index (BMI). Sperm DNA fragmentation (sperm chromatin structure analysis), sperm apoptosis (annexin V), and sperm apoptosis-related factors (antibody array assay) were assessed and their relationships with BMI were analyzed.

Results: BMI was not significantly related to age, duration of infertility, duration of sexual abstinence, semen volume, sperm concentration, or rate of normal sperm morphology (p > 0.05). However, progressive sperm motility was significantly reduced and the rates of sperm DNA fragmentation index (DFI) and sperm apoptosis were significantly increased in overweight and obese men compared with men with normal BMI. Fas/Fasl, Bcl-2/Bax, caspase-3, caspase-8, p53, and p21 were all upregulated in the overweight and obese groups. Protein function annotation by Gene Ontology analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis revealed that apoptosis-related factors were enriched in a network associated with activation of apoptotic signaling pathways, such as apoptosis and p53 signaling.

Conclusion: These data suggest that increased BMI is associated with increased sperm apoptosis and sperm DNA damage, as well as accelerated expression of apoptosis-related factors via the activation of apoptotic signaling pathways.

Keywords: BMI; apoptosis-related factors; biomarker; obesity; semen quality; sperm DNA.

Grants and funding

This work was supported by the joint construction fund of Henan Provincial Medical Science and Technology Tackling Program of China (LHGJ20191362). The funding body was not involved in the design of the study, the collection, analysis, and interpretation of data and the preparation of the manuscript.