Review of Stimulated Raman Scattering Microscopy Techniques and Applications in the Biosciences

Yanping Li; Binglin Shen; Shaowei Li; Yihua Zhao; Junle Qu; Liwei Liu

doi:10.1002/adbi.202000184

Review of Stimulated Raman Scattering Microscopy Techniques and Applications in the Biosciences

Adv Biol (Weinh). 2021 Jan;5(1):e2000184. doi: 10.1002/adbi.202000184. Epub 2020 Dec 30.

Authors

Yanping Li¹, Binglin Shen¹, Shaowei Li¹, Yihua Zhao¹, Junle Qu¹, Liwei Liu¹

Affiliation

¹ Key Laboratory of Optoelectronic Devices and Systems of Guangdong Province and Ministry of Education, College of Physics and Optoelectronic Engineering, Shenzhen University, 3688 Nanhai Avenue, Shenzhen, 518060, China.

PMID: 33724734
DOI: 10.1002/adbi.202000184

Abstract

Stimulated Raman scattering (SRS) microscopy is a nonlinear optical imaging method for visualizing chemical content based on molecular vibrational bonds. Featuring high speed, high resolution, high sensitivity, high accuracy, and 3D sectioning, SRS microscopy has made tremendous progress toward biochemical information acquisition, cellular function investigation, and label-free medical diagnosis in the biosciences. In this review, the principle of SRS, system design, and data analysis are introduced, and the current innovations of the SRS system are reviewed. In particular, combined with various bio-orthogonal Raman tags, the applications of SRS microscopy in cell metabolism, tumor diagnosis, neuroscience, drug tracking, and microbial detection are briefly examined. The future prospects for SRS microscopy are also shared.

Keywords: Raman scattering microscopy; Raman tags; biological imaging; chemical bond vibration; medical diagnosis.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Microscopy*
Nonlinear Optical Microscopy*
Spectrum Analysis, Raman
Vibration