Variation of homologous recombination in Populus tomentosa with different genotypes

Yi Chuan. 2021 Feb 16;43(2):182-193. doi: 10.16288/j.yczz.20-205.

Authors

Geng Xining¹, Lu Te², Du Kang², Yang Jun², Kang Xiangyang²

Affiliations

¹ Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China;National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China;Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing 100083, China;Henan Province Key Laboratory of Germplasm Innovation and Utilization of Eco-economic Woody Plant, Pingdingshan University, Pingdingshan 467000, China.
² Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, Beijing Forestry University, Beijing 100083, China;National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China;Beijing Laboratory of Urban and Rural Ecological Environment, Beijing Forestry University, Beijing 100083, China.

PMID: 33724220
DOI: 10.16288/j.yczz.20-205

Abstract
in English, Chinese

Homologous recombination is an important source of biological genetic variation. Limited by detection methods, there are only a few reports on the homologous recombination in high plants and its product - heteroduplex DNA (hDNA). In the present study, applying the strategy of detecting hDNA by constructing populations from inhibited post-meiotic segregation, two hybrid triploid populations were constructed from two maternal parents inPopulus tomentosa by inhibiting post-meiotic segregation. One hundred and ten simple sequence repeat (SSR) markers were used to study the occurrence and variation of hDNA on nine chromosomes inP. tomentosa with different genotypes. The results showed that the frequencies of hDNA between two female parents inP. tomentosa ranged from 8.5% to 87.2%. The hDNA frequency was positively correlated to the distance from the centromere, but the average hDNA frequency on a chromosome had no correlation with the chromosome length. One to 3 times recombination events were detected on most chromosomes, and only a few four- or five-times recombination events were detected. The overall frequencies of hDNA on the same chromosome in two genotypic individuals were roughly similar, while the hDNA frequencies varied greatly at specific SSR loci. Compared withTacamahaca poplar hybrid,P. pseudo-simonii × P. nigra 'Zheyin3#', detection of homologous recombination times and the frequency and location of hDNA were largely different. This study is the first to describe the characteristics and variations of homologous recombination inP. tomentosa with two different genotypes, which will provide valuable insights for exploring the characteristics and variations of homologous recombination among interspecies and intraspecies in higher plant.

同源重组是生物遗传变异的重要来源。受检测方法限制,高等植物同源重组发生及其产物——异源双链DNA(heteroduplex DNA, hDNA)鲜有报道。本研究采用构建抑制减数后分离群体检测同源重组产物hDNA的方法,以2个母本来源的基于抑制减数后分离获得的毛白杨(Populus tomentosa)杂种三倍体群体为研究材料,利用筛选出的110个简单序列重复(Simple sequence repeat, SSR)分子标记开展毛白杨不同基因型个体间9条染色体上hDNA发生及其遗传变异研究。结果表明,2个毛白杨雌株hDNA发生频率介于8.5%~87.2%之间,且hDNA发生频率与距着丝粒距离呈正相关关系,但同一染色体平均hDNA发生频率与染色体长度无相关关系;绝大多数的染色体检测出1~3次重组事件,少数检测出4次重组事件,极少数检测到5次重组事件;不同毛白杨基因型个体间同一染色体上hDNA发生频率总体相差不大,而在一些特定SSR位点间hDNA发生频率存在较大差异;与青杨杂种‘哲引3号’杨(P. pseudo-simonii×P. nigra ‘Zheyin3#’)相比,检测到的同源重组次数及hDNA发生频率和发生位置均存在较大差异。本研究首次对2个基因型毛白杨同源重组发生特征及其变异进行了研究,为揭示高等植物同源重组特点、种间和种内同源重组差异等提供了重要见解。.

Keywords: Populus tomentosa; genotype; heteroduplex DNA; homologous recombination; triploid.

MeSH terms

Female
Genotype
Homologous Recombination
Humans
Microsatellite Repeats / genetics
Nucleic Acid Heteroduplexes
Populus* / genetics

Substances

Nucleic Acid Heteroduplexes