Rapid detection of fungi from blood samples of patients with candidemia using modified calcofluor white stain

Biao Chen; Wenqiang Li; Yin Pang; Ning Zhang; Shaodong Bian; Chen Liu; Zhiya Yang; Yufeng Jiang; Renzhe Li; Yingguang Xie; Dongmei Shi

doi:10.1016/j.mimet.2021.106202

Rapid detection of fungi from blood samples of patients with candidemia using modified calcofluor white stain

J Microbiol Methods. 2021 May:184:106202. doi: 10.1016/j.mimet.2021.106202. Epub 2021 Mar 12.

Authors

Biao Chen¹, Wenqiang Li², Yin Pang³, Ning Zhang⁴, Shaodong Bian⁴, Chen Liu⁴, Zhiya Yang⁴, Yufeng Jiang⁵, Renzhe Li⁶, Yingguang Xie⁷, Dongmei Shi⁸

Affiliations

¹ Shandong University of Traditional Chinese Medicine, Jinan 210355, Shandong, China; The Laboratory of Medical Mycology, Jining No. 1 People's Hospital, Jining 272111, Shandong, China.
² Intensive Care Unit, Jining No. 1 People's Hospital, Jining 272111, Shandong, China.
³ Department of Pediatrics, Jining No. 1 People's Hospital, Jining 272111, Shandong, China.
⁴ The Laboratory of Medical Mycology, Jining No. 1 People's Hospital, Jining 272111, Shandong, China.
⁵ Shandong University of Traditional Chinese Medicine, Jinan 210355, Shandong, China; Department of Medical Laboratory, Jining No. 1 People's Hospital, Jining 272111, Shandong, China.
⁶ Department of Medical Laboratory, Jining No. 1 People's Hospital, Jining 272111, Shandong, China.
⁷ Intensive Care Unit, Jining No. 1 People's Hospital, Jining 272111, Shandong, China. Electronic address: zzyxkx@163.com.
⁸ The Laboratory of Medical Mycology, Jining No. 1 People's Hospital, Jining 272111, Shandong, China; Department of Dermatology, Jining No. 1 People's Hospital, Jining, Shandong, China. Electronic address: shidongmei28@163.com.

PMID: 33722638
DOI: 10.1016/j.mimet.2021.106202

Abstract

We reported a modified CFW assay for rapid detection of fungi in blood samples and evaluated its efficacy in vivo and in vitro. The positive rate, sensitivity, and negative predictive values of the modified CFW method were all significantly higher than those of traditional fungal culture and KOH methods.

Keywords: Blood; Candida albicans; Candidemia; Modified calcofluor white stain.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Benzenesulfonates / chemistry
Blood / microbiology
Candidemia / blood
Candidemia / diagnosis
Candidemia / microbiology*
Diagnostic Tests, Routine / methods
Female
Fungi / chemistry
Fungi / isolation & purification*
Humans
Mice
Sensitivity and Specificity
Staining and Labeling / methods*

Substances

Benzenesulfonates
C.I. Fluorescent Brightening Agent 28