Exploiting the photoactivity of bacterial reaction center to investigate liposome dynamics

Emiliano Altamura; Francesco Milano; Pasquale Stano; Fabio Mavelli

doi:10.1007/s43630-021-00011-3

Exploiting the photoactivity of bacterial reaction center to investigate liposome dynamics

Photochem Photobiol Sci. 2021 Feb;20(2):321-326. doi: 10.1007/s43630-021-00011-3. Epub 2021 Feb 10.

Authors

Emiliano Altamura¹, Francesco Milano², Pasquale Stano³, Fabio Mavelli⁴

Affiliations

¹ Department of Chemistry, Università Degli Studi Di Bari "Aldo Moro", Via Orabona 4, 70125, Bari, Italy.
² Institute of Science of Food Production (ISPA), CNR, Str. Provinciale Lecce-Monteroni, 73100, Lecce, Italy.
³ Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Salento, Str. Provinciale Lecce-Monteroni, 73100, Lecce, Italy.
⁴ Department of Chemistry, Università Degli Studi Di Bari "Aldo Moro", Via Orabona 4, 70125, Bari, Italy. fabio.mavelli@uniba.it.

PMID: 33721250
DOI: 10.1007/s43630-021-00011-3

Abstract

Charge recombination kinetics of bacterial photosynthetic protein Reaction Center displays an exquisite sensitivity to the actual occupancy of ubiquinone-10 in its Q_B-binding site. Here, we have exploited such phenomenon for assessing the growth and the aggregation/fusion of phosphocholine vesicles embedding RC in their membrane, when treated with sodium oleate.

MeSH terms

Bacterial Proteins / chemistry*
Bacterial Proteins / metabolism
Dynamic Light Scattering
Liposomes / chemistry*
Membrane Fusion / drug effects
Oleic Acid / chemistry
Oleic Acid / pharmacology
Photosynthetic Reaction Center Complex Proteins / chemistry*
Photosynthetic Reaction Center Complex Proteins / metabolism
Rhodobacter sphaeroides / metabolism*
Ubiquinone / analogs & derivatives
Ubiquinone / chemistry
Ubiquinone / metabolism

Substances

Bacterial Proteins
Liposomes
Photosynthetic Reaction Center Complex Proteins
Ubiquinone
Oleic Acid
osteum
coenzyme Q10