GhPIPLC2D promotes cotton fiber elongation by enhancing ethylene biosynthesis

Liping Zhu; Lingling Dou; Haihong Shang; Hongbin Li; Jianing Yu; Guanghui Xiao

doi:10.1016/j.isci.2021.102199

GhPIPLC2D promotes cotton fiber elongation by enhancing ethylene biosynthesis

iScience. 2021 Feb 17;24(3):102199. doi: 10.1016/j.isci.2021.102199. eCollection 2021 Mar 19.

Authors

Liping Zhu¹, Lingling Dou², Haihong Shang³, Hongbin Li⁴, Jianing Yu¹, Guanghui Xiao¹

Affiliations

¹ College of Life Sciences, Shaanxi Normal University, Xi'an 710119, China.
² School of Chemistry and Chemical Engineering, Xianyang Normal University, Xianyang 712000, China.
³ Zhengzhou Research Base, State Key Laboratory of Cotton Biology, Zhengzhou University, Zhengzhou 450000, China.
⁴ College of Life Sciences, Key Laboratory of Xinjiang Phytomedicine Resource and Utilization of Ministry of Education, Shihezi University, Shihezi 832003, China.

Abstract

Inositol-1,4,5-trisphosphate (IP₃) is an important second messenger and one of the products of phosphoinositide-specific phospholipase C (PIPLC)-mediated phosphatidylinositol (4,5) bisphosphate (PIP₂) hydrolysis. However, the function of IP₃ in cotton is unknown. Here, we characterized the function of GhPIPLC2D in cotton fiber elongation. GhPIPLC2D was preferentially expressed in elongating fibers. Suppression of GhPIPLC2D transcripts resulted in shorter fibers and decreased IP₃ accumulation and ethylene biosynthesis. Exogenous application of linolenic acid (C18:3) and phosphatidylinositol (PI), the precursor of IP₃, improved IP₃ and myo-inositol-1,2,3,4,5,6-hexakisphosphate (IP₆) accumulation, as well as ethylene biosynthesis. Moreover, fiber length in GhPIPLC2D-silenced plant was reduced after exogenous application of IP₆ and ethylene. These results indicate that GhPIPLC2D positively regulates fiber elongation and IP₃ promotes fiber elongation by enhancing ethylene biosynthesis. Our study broadens our understanding of the function of IP₃ in cotton fiber elongation and highlights the possibility of cultivating better cotton varieties by manipulating GhPIPLC2D in the future.

Keywords: biological sciences; plant biology; plant development.