Hydrogen peroxide (H2O2), an important representatives of reactive oxygen species, its aberrant expression is related to many diseases, including cancers. Therefore, it is very significant to design a reliable method for the real-time detection of endogenous H2O2. Herein, we describe preparing a poly (azure I)/crumpled graphene (cGN) modified electrode by an electro-polymerization method. The results showed that this electrode presented obvious electrocatalytic effect on the reduction of H2O2. Amperometric method was employed to monitor H2O2, and the amperometric response exhibited a good linear relationship with its concentration in the range of 8.0 × 10-6 - 1.25 × 10-3 mol/L; the detection limit reached to 6.7 × 10-7 mol/L (S/N = 3). Furthermore, this modified also displayed good selectivity, long-term stability and a high anti-interference ability. Excitingly, the established method could be successfully used to detect H2O2 in human serum samples, and measured H2O2 secreted from living MCF-7 cells. It could have potential use in cancer diagnosis.
Keywords: Graphene; hydrogen peroxide; modified electrode; poly (azure I).