In vitro assessment of the efficacy of protein exudates from seeds against Haemonchus contortus

Irlla Correia Lima Licá; Alexandra Martins Santos Soares; André Teixeira Silva Ferreira; Jonas Perales; Ivo Alexandre Leme Cunha; Pedro Filho Noronha Souza; Livio Martins Costa-Júnior

doi:10.1016/j.vetpar.2021.109399

In vitro assessment of the efficacy of protein exudates from seeds against Haemonchus contortus

Vet Parasitol. 2021 Apr:292:109399. doi: 10.1016/j.vetpar.2021.109399. Epub 2021 Mar 5.

Authors

Irlla Correia Lima Licá¹, Alexandra Martins Santos Soares², André Teixeira Silva Ferreira³, Jonas Perales³, Ivo Alexandre Leme Cunha⁴, Pedro Filho Noronha Souza⁵, Livio Martins Costa-Júnior⁶

Affiliations

¹ Postgraduate Program in Health Sciences, Federal University of Maranhão, São Luís, MA, Brazil; Laboratory of Plant Biochemistry, Federal University of Maranhão, São Luís, MA, Brazil.
² Postgraduate Program in Health Sciences, Federal University of Maranhão, São Luís, MA, Brazil; Laboratory of Plant Biochemistry, Federal University of Maranhão, São Luís, MA, Brazil. Electronic address: alexanddra.ufma@gmail.com.
³ Laboratory of Toxinology, Oswaldo Cruz Foundation, Rio de Janeiro, Brazil.
⁴ Laboratory of Applied Parasitology, Federal University of Maranhão, Chapadinha, MA, Brazil.
⁵ Center for Virology, University of Nebraska Lincoln, Lincoln, NE, United States.
⁶ Postgraduate Program in Health Sciences, Federal University of Maranhão, São Luís, MA, Brazil; Laboratory of Parasite Control, Federal University of Maranhão, São Luís, MA, Brazil. Electronic address: livio.martins@ufma.br.

PMID: 33711619
DOI: 10.1016/j.vetpar.2021.109399

Abstract

Nematodes develop resistance to the most common commercially available drugs. The aim of this study was to identify and evaluate the action of protein exudates from Mimosa caesalpiniifolia, Leucaena leucocephala, Acacia mangium, and Stylosanthes capitata seeds on the gastrointestinal nematode Haemonchus contortus. The exuded proteins were precipitated, dialyzed, lyophilized, and assessed for their effect on egg hatching and artificial larval exsheathment inhibition. Proteome analysis of the protein extracts was also performed. Although no egg-hatching inhibition was observed, all exudates showed efficacy in inhibiting the larval exsheathment of H. contortus larvae with an EC₅₀ varying from 0.61 to 0.26 mg P mL^-1. Proteomic analysis revealed the presence of proteases, protease inhibitors, chitinases, and lectins among other proteins in the exudates. Most of the exuded proteins belong to the oxidative stress/plant defense and energy/carbohydrate metabolism functional clusters. This study concluded that the bioactive proteins from different classes exuded by seeds of M. caesalpiniifolia, L. leucocephala, A. mangium, and S. capitata show stage-specific inhibition against H. contortus.

Keywords: Egg Hatching; Exsheathment; Nematode; Plant defense proteins; Proteome.

MeSH terms

Animals
Anthelmintics / chemistry
Anthelmintics / pharmacology
Exudates and Transudates / chemistry*
Fabaceae / chemistry*
Haemonchus / drug effects*
Plant Exudates / chemistry
Plant Proteins / pharmacology*
Seeds / chemistry*

Substances

Anthelmintics
Plant Exudates
Plant Proteins