A novel actinobacterium, designated strain K274T, was isolated from soil collected from Zafer Cape (Cape Apostolos Andreas), the easternmost tip of Cyprus on the Karpas peninsula, Magusa, Northern Cyprus, and a polyphasic approach was used for characterization of the strain. The isolate was found to have chemotaxonomic and morphological properties associated with members of the genus Nonomuraea. The strain has the highest similarity to Nonomuraea zeae DSM 100528T with 99.1% similarity value. In the phylogenetic dendogram based on 16S rRNA gene sequence, strain K274T was formed a distinct clade together N. zeae DSM 100528T, 'Nonomuraea basaltis' 160415 (98.9% similarity), and 'Nonomuraea lycopersici' NEAU-DE8(1) (98.2% similarity). The genome sequence of strain K274T was 11.5 Mbp in size with a total of 11,848 protein-coding genes and 75 RNA genes. The genomic G + C content of the novel strain was 69.7 mol%. Both average nucleotide identity (ANI) and digital DNA-DNA hybridization (dDDH) results between the strain and phlyogenetic neighbours were well below the threshold value, and the novelty are supported by phenotypic and chemotaxonomic differences. Because of all these, strain K274T represents a novel species in the genus Nonomuraea, for which the name Nonomuraea cypriaca sp. nov. is proposed. The type strain is K274T (= DSM 45718T = KCTC 29095T).
Keywords: 16S rRNA gene; Nonomuraea cypriaca sp. nov.; Polyphasic taxonomy.