Characterization of novel Gluconobacter species from fruits and fermented food products : Gluconobacter cadivus sp. nov. , Gluconobacter vitians sp. nov. and Gluconobacter potus sp. nov

Atena Sadat Sombolestani; Ilse Cleenwerck; Margo Cnockaert; Wim Borremans; Anneleen D Wieme; Luc De Vuyst; Peter Vandamme

doi:10.1099/ijsem.0.004751

Characterization of novel Gluconobacter species from fruits and fermented food products : Gluconobacter cadivus sp. nov. , Gluconobacter vitians sp. nov. and Gluconobacter potus sp. nov

Int J Syst Evol Microbiol. 2019 Jun;71(3). doi: 10.1099/ijsem.0.004751. Epub 2021 Mar 12.

Authors

Atena Sadat Sombolestani¹, Ilse Cleenwerck², Margo Cnockaert¹, Wim Borremans³, Anneleen D Wieme², Luc De Vuyst³, Peter Vandamme^{2

1}

Affiliations

¹ Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
² BCCM/LMG Bacteria Collection, Laboratory of Microbiology, Department of Biochemistry and Microbiology, Faculty of Sciences, Ghent University, K. L. Ledeganckstraat 35, B-9000 Ghent, Belgium.
³ Research Group of Industrial Microbiology and Food Biotechnology, Department of Bioengineering Sciences, Faculty of Sciences and Bioengineering Sciences, Vrije Universiteit Brussel, Pleinlaan 2, B-1050 Brussels, Belgium.

PMID: 33709905
DOI: 10.1099/ijsem.0.004751

Abstract

Strains LMG 1744^T, LMG 1745, LMG 31484^T, LMG 1764^T and R-71646 were isolated from rotting fruits and fermented food products. A phylogenomic analysis based on 107 single-copy core genes revealed that they grouped in a Gluconobacter lineage comprising Gluconobacter oxydans, Gluconobacter roseus, Gluconobacter sphaericus, Gluconobacter kanchanaburiensis, Gluconobacter albidus, Gluconobacter cerevisiae, Gluconobacter kondonii and Gluconobacter aidae. OrthoANIu and digital DNA hybridization analyses demonstrated that these five strains represented three novel Gluconobacter species, which could be differentiated from the type strains of closely related Gluconobacter species by multiple phenotypic characteristics. We therefore propose to classify strains LMG 1744^T and LMG 1745 in the novel species Gluconobacter cadivus sp. nov., with LMG 1744^T (=CECT 30141^T) as the type strain; to classify strain LMG 31484^T as the novel species Gluconobacter vitians sp. nov., with LMG 31484^T (=CECT 30132^T) as the type strain; and to classify strains LMG 1764^T and R-71646 in the novel species Gluconobacter potus sp. nov., with LMG 1764^T (=CECT 30140^T) as the type strain.

Keywords: Gluconobacter; OrthoANIu; acetic acid bacteria; beer; digital DNA-DNA hybridization; fruit; kombucha.