Minor contribution of NCX to Na+-Ca2+ exchange activity in brain mitochondria

Abstract

NCLX was identified as a mitochondrial Na⁺-Ca²⁺ exchanger. However, contribution of NCLX to overall mitochondrial Na⁺-Ca²⁺ exchange activity remains unclear, especially in brain mitochondria where plasma membrane Na⁺-Ca²⁺ exchanger NCX also exists. We studied the issue using isolated mouse brain mitochondria. The Na⁺- as well as Li⁺-dependent Ca²⁺ efflux from mitochondria was significantly inhibited by a NCLX blocker, but was insensitive to NCX blockers, suggesting that NCLX comprises a major part in forward mode of mitochondrial Na⁺-Ca²⁺ exchange activity. On the other hand, the Na⁺-dependent Ca²⁺ influx into mitochondria, the reverse mode, was insensitive to all the blockers tested, suggesting unidentified Ca²⁺ transport systems.

Keywords: Brain; Mitochondria; NCLX; NCX.