PARP inhibition suppresses topoisomerase 1 poisoning induced Caspase-3 dependent cell death in zebrafish embryos

Biochem Biophys Res Commun. 2021 Apr 23:550:166-170. doi: 10.1016/j.bbrc.2021.03.005. Epub 2021 Mar 8.

Abstract

In the present study the role of poly(ADP)ribosylation on rubitecan induced caspase dependent cell death was evaluated. We show that Top1 poisoning by rubitecan induces caspase mediated apoptosis which was reduced by PARP inhibitor olaparib in zebrafish embryo. Collectively our data introduces zebrafish as a valuable model for PARP related biomedical research.

Keywords: Caspase-3; Olaparib; PARP-1; Rubitecan; Zebrafish embryo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Apoptosis / drug effects*
  • Camptothecin / analogs & derivatives
  • Camptothecin / pharmacology
  • Caspase 3 / metabolism*
  • DNA Topoisomerases, Type I / metabolism*
  • Drug Synergism
  • Phthalazines / pharmacology
  • Piperazines / pharmacology
  • Poly (ADP-Ribose) Polymerase-1 / analysis
  • Poly (ADP-Ribose) Polymerase-1 / chemistry
  • Poly (ADP-Ribose) Polymerase-1 / metabolism*
  • Poly(ADP-ribose) Polymerase Inhibitors / pharmacology*
  • Topoisomerase I Inhibitors / pharmacology*
  • Zebrafish / embryology*

Substances

  • Phthalazines
  • Piperazines
  • Poly(ADP-ribose) Polymerase Inhibitors
  • Topoisomerase I Inhibitors
  • Poly (ADP-Ribose) Polymerase-1
  • Caspase 3
  • DNA Topoisomerases, Type I
  • rubitecan
  • olaparib
  • Camptothecin