A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells

Jelena Grahovac; Marijana Pavlović; Marija Ostojić

doi:10.1007/978-1-0716-1205-7_5

A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells

Methods Mol Biol. 2021:2265:73-80. doi: 10.1007/978-1-0716-1205-7_5.

Authors

Jelena Grahovac¹, Marijana Pavlović², Marija Ostojić²

Affiliations

¹ Experimental Oncology Department, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia. jelena.grahovac@ncrc.ac.rs.
² Experimental Oncology Department, Institute for Oncology and Radiology of Serbia, Belgrade, Serbia.

PMID: 33704706
DOI: 10.1007/978-1-0716-1205-7_5

Abstract

Melanoma cells have high glycolytic capacity. Glucose uptake is a key rate-limiting step in glucose utilization. Here we describe a simple protocol for measuring direct glucose uptake in living melanoma cells by flow cytometry.

Keywords: 2-NBDG; Flow cytometry; Glucose uptake; Melanoma.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

4-Chloro-7-nitrobenzofurazan / analogs & derivatives
4-Chloro-7-nitrobenzofurazan / chemistry
Biological Transport
Cell Culture Techniques / methods
Cell Line, Tumor
Deoxyglucose / analogs & derivatives
Deoxyglucose / chemistry
Flow Cytometry / methods*
Fluorescence
Fluorescent Dyes / chemistry
Glucose / metabolism*
Humans
Melanoma / metabolism*

Substances

Fluorescent Dyes
Deoxyglucose
4-Chloro-7-nitrobenzofurazan
Glucose
2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose