Detection of feline norovirus using commercial real-time RT-PCR kit for the diagnosis of human norovirus infection

J Vet Med Sci. 2021 May 9;83(5):805-808. doi: 10.1292/jvms.20-0703. Epub 2021 Mar 11.

Abstract

Feline noroviruses (FNoVs) are potential clinical pathogens in cats. To perform an epidemiological study of FNoV infection, it is necessary to develop a simple and effective method for virus detection. We investigated whether a commercial human NoV quantitative RT-PCR kit for the detection of human NoVs used in medical practice can be applied for FNoV detection. This kit was capable of detecting the FNoV gene regardless of the genogroup (GIV and GVI) in experimental and field samples. Based on the above findings, it is possible to detect FNoVs using human NoV tests. The relationship between FNoV infection and gastroenteritis in cats may be clarified by applying these methods to an epidemiological survey of FNoVs.

Keywords: commercial human kit; diagnostic method; feline norovirus (FNoV); genogroup.

MeSH terms

  • Animals
  • Caliciviridae Infections* / diagnosis
  • Caliciviridae Infections* / epidemiology
  • Caliciviridae Infections* / veterinary
  • Cat Diseases* / diagnosis
  • Cat Diseases* / epidemiology
  • Cats
  • Feces
  • Gastroenteritis* / diagnosis
  • Gastroenteritis* / epidemiology
  • Gastroenteritis* / veterinary
  • Genotype
  • Humans
  • Norovirus* / genetics
  • Real-Time Polymerase Chain Reaction / veterinary
  • Reverse Transcriptase Polymerase Chain Reaction / veterinary