Incoherent color digital holography with computational coherent superposition for fluorescence imaging [Invited]

Appl Opt. 2021 Feb 1;60(4):A260-A267. doi: 10.1364/AO.406068.

Abstract

We present color fluorescence imaging using an incoherent digital holographic technique in which holographic multiplexing of multiple wavelengths is exploited. Self-interference incoherent digital holography with a single-path in-line configuration and the computational coherent superposition scheme are adopted to obtain color holographic three-dimensional information of self-luminous objects with a monochrome image sensor and no mechanical scanning. We perform not only simultaneous color three-dimensional sensing of multiple self-luminous objects but also color fluorescence imaging of stained biological samples. Color fluorescence imaging with an improved point spread function is also demonstrated experimentally by adopting a Fresnel incoherent correlation holography system.

MeSH terms

  • Coordination Complexes / chemistry
  • Equipment Design
  • Europium / chemistry
  • Fluorescent Dyes / chemistry
  • HeLa Cells
  • Holography / instrumentation*
  • Holography / methods
  • Humans
  • Image Processing, Computer-Assisted
  • Optical Imaging / instrumentation*
  • Optical Imaging / methods
  • Terbium / chemistry

Substances

  • Coordination Complexes
  • Fluorescent Dyes
  • Terbium
  • Europium