Abstract
Herein, we applied PmST1 (a sialyltransferase) to achieve acceptor-mediated regioselective sialylation (AMRS) on the nonreducing end GalNH2 or GalAz (2-azido-2-deoxy galactose). Thus, C5 and C8-modified sialic acid was efficiently assembled on GalNH2 (or GalAz) to achieve the synthesis of the GAA-7 (one of the echinodermatous gangliosides with higher neuritogenic activity) glycan moiety.
MeSH terms
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Biocatalysis
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Carbohydrate Conformation
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Gangliosides / biosynthesis*
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Gangliosides / chemistry
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N-Acetylneuraminic Acid / chemistry
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N-Acetylneuraminic Acid / metabolism*
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Polysaccharides / biosynthesis*
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Polysaccharides / chemistry
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Sialyltransferases / metabolism*
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Stereoisomerism
Substances
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Gangliosides
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Polysaccharides
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ganglioside GAA-7
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Sialyltransferases
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N-Acetylneuraminic Acid