Adipose tissue-derived mesenchymal stem cells (AD-MSCs) offer great therapeutic potential for osteoarthritis (OA) treatment. Recent investigations have revealed the contribution of extracellular vesicles (EVs) to AD-MSC actions. Here, we describe a method to study the in vitro effects of EVs from AD-MSCs in OA chondrocytes. This chapter includes the isolation and analysis of human AD-MSCs and their EVs as well as the isolation and treatment of OA chondrocytes.
Keywords: Adipose tissue-derived mesenchymal stem cells; Chondrocytes; Exosomes; Extracellular vesicles; Microvesicles; Osteoarthritis; Tunable Resistive Pulse Sensing.