Evaluation of Extracellular Vesicles from Adipose Tissue-Derived Mesenchymal Stem Cells in Primary Human Chondrocytes from Patients with Osteoarthritis

Methods Mol Biol. 2021:2269:221-231. doi: 10.1007/978-1-0716-1225-5_15.

Abstract

Adipose tissue-derived mesenchymal stem cells (AD-MSCs) offer great therapeutic potential for osteoarthritis (OA) treatment. Recent investigations have revealed the contribution of extracellular vesicles (EVs) to AD-MSC actions. Here, we describe a method to study the in vitro effects of EVs from AD-MSCs in OA chondrocytes. This chapter includes the isolation and analysis of human AD-MSCs and their EVs as well as the isolation and treatment of OA chondrocytes.

Keywords: Adipose tissue-derived mesenchymal stem cells; Chondrocytes; Exosomes; Extracellular vesicles; Microvesicles; Osteoarthritis; Tunable Resistive Pulse Sensing.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipose Tissue / metabolism*
  • Adipose Tissue / pathology
  • Cell Separation
  • Chondrocytes / metabolism*
  • Chondrocytes / pathology
  • Coculture Techniques
  • Extracellular Vesicles / metabolism*
  • Humans
  • Mesenchymal Stem Cells / metabolism*
  • Mesenchymal Stem Cells / pathology
  • Osteoarthritis / metabolism*
  • Osteoarthritis / pathology