Fluorescent imaging in living animals gives an intuitive picture of the dynamic processes in the complex environment within a living being. However, animal tissues present a substantial barrier and are opaque to most wavelengths of visible light. Fluorescent nanoparticles (NPs) with new photophysical characteristics have shown excellent performance for in vivo imaging. Hence, fluorescent NPs have been widely studied and applied for the detection of molecular and biological processes in living animals. In addition, developments in the area of nanotechnology have allowed materials to be used in intact animals for disease detection, diagnosis, drug delivery, and treatment. This review provides information on the different types of fluorescent particles based on nanotechnology, describing their unique individual properties and applications for detecting vital processes in vivo. The development and application of new fluorescent NPs will provide opportunities for in vivo imaging with better penetration, sensitivity, and resolution. This article is categorized under: Diagnostic Tools > in vivo Nanodiagnostics and Imaging.
Keywords: fluorescent material; in vivo imaging; multimodality imaging; nanotechnology.
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